Publikation: jYCaMP : an optimized calcium indicator for two-photon imaging at fiber laser wavelengths
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Datum
2020
Autor:innen
Mohr, Manuel Alexander
Bushey, Daniel
Aggarwal, Abhi
Marvin, Jonathan S.
Kim, Jeong Jun
Marquez, Emiliano Jimenez
Liang, Yajie
Patel, Ronak
Podgorski, Kaspar
et al.
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U.S. National Science Foundation (NSF): 1707287
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Open Access-Veröffentlichung
Core Facility der Universität Konstanz
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Published
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Nature Methods. Springer. 2020, 17(7), S. 694-697. ISSN 1548-7091. eISSN 1548-7105. Verfügbar unter: doi: 10.1038/s41592-020-0835-7
Zusammenfassung
Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Fluorescence imaging, Fluorescent proteins
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MOHR, Manuel Alexander, Daniel BUSHEY, Abhi AGGARWAL, Jonathan S. MARVIN, Jeong Jun KIM, Emiliano Jimenez MARQUEZ, Yajie LIANG, Ronak PATEL, Chi-Yu LEE, Kaspar PODGORSKI, 2020. jYCaMP : an optimized calcium indicator for two-photon imaging at fiber laser wavelengths. In: Nature Methods. Springer. 2020, 17(7), S. 694-697. ISSN 1548-7091. eISSN 1548-7105. Verfügbar unter: doi: 10.1038/s41592-020-0835-7BibTex
@article{Mohr2020-07jYCaM-73439,
title={jYCaMP : an optimized calcium indicator for two-photon imaging at fiber laser wavelengths},
year={2020},
doi={10.1038/s41592-020-0835-7},
number={7},
volume={17},
issn={1548-7091},
journal={Nature Methods},
pages={694--697},
author={Mohr, Manuel Alexander and Bushey, Daniel and Aggarwal, Abhi and Marvin, Jonathan S. and Kim, Jeong Jun and Marquez, Emiliano Jimenez and Liang, Yajie and Patel, Ronak and Lee, Chi-Yu and Podgorski, Kaspar}
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