BDNF increases the number of axotomized rat retinal ganglion cells expressing GAP-43, L1, and TAG-1 mRNA--a supportive role for nitric oxide?

Klöcker, Nikolaj; Jung, Marion; Stürmer, Claudia; Bähr, Mathias

BDNF increases the number of axotomized rat retinal ganglion cells expressing GAP-43, L1, and TAG-1 mRNA--a supportive role for nitric oxide?

Files

Kloecker_174748.pdf(280.74 KB)

Date

2001

Authors

Klöcker, Nikolaj

Jung, Marion

Stürmer, Claudia

Bähr, Mathias

Publication type

Journal article

Published in

Neurobiology of Disease ; 8 (2001), 1. - pp. 103-113. - ISSN 0969-9961

Abstract

The death of neurons and the limited ability to activate growth-associated genes prevent the restoration of lesioned fiber tracts in the adult mammalian CNS. Here, we characterized the effects of the survival-promoting neurotrophin brain-derived neurotrophic factor (BDNF) on mRNA expression of GAP-43, L1, TAG-1, and SC-1 in axotomized and regenerating rat retinal ganglion cells (RGCs). BDNF led to de novo upregulation of TAG-1 mRNA in axotomized RGCs and to a threefold increase in the number of GAP-43 and L1 mRNA-expressing RGCs. SC-1 expression remained unchanged. However, BDNF did not improve long-distance axon regeneration into a peripheral nerve graft. Surprisingly, potentiating BDNF-mediated neuroprotection by simultaneous administration of a spin trap or a NOS inhibitor counteracted the BDNF-induced growth-associated gene expression. This led us to hypothesize that the BDNF effects on GAP-43, L1, and TAG-1 mRNA expression are mediated by a NO-dependent mechanism. In summary, our data support the idea that survival and axon regeneration of lesioned CNS neurons can be regulated independently.

Subject (DDC)

570 Biosciences, Biology

Cite This

ISO 690

KLÖCKER, Nikolaj, Marion JUNG, Claudia STÜRMER, Mathias BÄHR, 2001. BDNF increases the number of axotomized rat retinal ganglion cells expressing GAP-43, L1, and TAG-1 mRNA--a supportive role for nitric oxide?. In: Neurobiology of Disease. 8(1), pp. 103-113. ISSN 0969-9961. Available under: doi: 10.1006/nbdi.2000.0329

BibTex

@article{Klocker2001-02incre-17474,
  year={2001},
  doi={10.1006/nbdi.2000.0329},
  title={BDNF increases the number of axotomized rat retinal ganglion cells expressing GAP-43, L1, and TAG-1 mRNA--a supportive role for nitric oxide?},
  number={1},
  volume={8},
  issn={0969-9961},
  journal={Neurobiology of Disease},
  pages={103--113},
  author={Klöcker, Nikolaj and Jung, Marion and Stürmer, Claudia and Bähr, Mathias}
}

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