BDNF increases the number of axotomized rat retinal ganglion cells expressing GAP-43, L1, and TAG-1 mRNA--a supportive role for nitric oxide?

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2001
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Klöcker, Nikolaj
Jung, Marion
Bähr, Mathias
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Neurobiology of Disease. 2001, 8(1), pp. 103-113. ISSN 0969-9961. Available under: doi: 10.1006/nbdi.2000.0329
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The death of neurons and the limited ability to activate growth-associated genes prevent the restoration of lesioned fiber tracts in the adult mammalian CNS. Here, we characterized the effects of the survival-promoting neurotrophin brain-derived neurotrophic factor (BDNF) on mRNA expression of GAP-43, L1, TAG-1, and SC-1 in axotomized and regenerating rat retinal ganglion cells (RGCs). BDNF led to de novo upregulation of TAG-1 mRNA in axotomized RGCs and to a threefold increase in the number of GAP-43 and L1 mRNA-expressing RGCs. SC-1 expression remained unchanged. However, BDNF did not improve long-distance axon regeneration into a peripheral nerve graft. Surprisingly, potentiating BDNF-mediated neuroprotection by simultaneous administration of a spin trap or a NOS inhibitor counteracted the BDNF-induced growth-associated gene expression. This led us to hypothesize that the BDNF effects on GAP-43, L1, and TAG-1 mRNA expression are mediated by a NO-dependent mechanism. In summary, our data support the idea that survival and axon regeneration of lesioned CNS neurons can be regulated independently.

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570 Biowissenschaften, Biologie
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ISO 690KLÖCKER, Nikolaj, Marion JUNG, Claudia STÜRMER, Mathias BÄHR, 2001. BDNF increases the number of axotomized rat retinal ganglion cells expressing GAP-43, L1, and TAG-1 mRNA--a supportive role for nitric oxide?. In: Neurobiology of Disease. 2001, 8(1), pp. 103-113. ISSN 0969-9961. Available under: doi: 10.1006/nbdi.2000.0329
BibTex
@article{Klocker2001-02incre-17474,
  year={2001},
  doi={10.1006/nbdi.2000.0329},
  title={BDNF increases the number of axotomized rat retinal ganglion cells expressing GAP-43, L1, and TAG-1 mRNA--a supportive role for nitric oxide?},
  number={1},
  volume={8},
  issn={0969-9961},
  journal={Neurobiology of Disease},
  pages={103--113},
  author={Klöcker, Nikolaj and Jung, Marion and Stürmer, Claudia and Bähr, Mathias}
}
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