Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici

Kurzanzeige
dc.contributor.authorAbt, Dietmar J.deu
dc.contributor.authorEinsle, Oliverdeu
dc.contributor.authorNiessen, Holgerdeu
dc.contributor.authorKrieger, Robertdeu
dc.contributor.authorMesserschmidt, Albrechtdeu
dc.contributor.authorSchink, Bernhard
dc.contributor.authorKroneck, Peter M. H.
dc.date.accessioned2011-03-24T17:33:29Zdeu
dc.date.available2011-03-24T17:33:29Zdeu
dc.date.issued2002deu
dc.description.abstractCrystals of the molybdo-/iron±sulfur protein pyrogallol:phloroglucinol hydroxyltransferase (transhydroxylase; EC 1.97.1.2) from Pelobacter acidigallici were grown by vapour diffusion in an N2/H2 atmosphere using polyethylene glycol as a precipitant. In this microorganism, transhydroxylase converts pyrogallol to phloroglucinol in a unique reaction without oxygen transfer from water.
Growth of crystals suitable for X-ray analysis was strongly dependent on the presence of dithionite as a reducing agent. The crystals belonged to space group P1 and MAD data were collected on the iron K edge to resolutions higher than 2.5 .		eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Acta Crystallographica / Section D, Biological Crystallography, 58 (2002), pp. 343-345deu
dc.identifier.ppn284138452deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/7317
dc.language.isoengdeu
dc.legacy.dateIssued2008deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subject.ddc570deu
dc.titleCrystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallicieng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
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@article{Abt2002Cryst-7317,
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  title={Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici},
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  volume={58},
  issn={0907-4449},
  journal={Acta Crystallographica},
  pages={343--345},
  author={Abt, Dietmar J. and Einsle, Oliver and Niessen, Holger and Krieger, Robert and Messerschmidt, Albrecht and Schink, Bernhard and Kroneck, Peter M. H.}
}
kops.citation.iso690ABT, Dietmar J., Oliver EINSLE, Holger NIESSEN, Robert KRIEGER, Albrecht MESSERSCHMIDT, Bernhard SCHINK, Peter M. H. KRONECK, 2002. Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici. In: Acta Crystallographica. 2002, 58(2), pp. 343-345. ISSN 0907-4449. eISSN 1399-0047deu
kops.citation.iso690ABT, Dietmar J., Oliver EINSLE, Holger NIESSEN, Robert KRIEGER, Albrecht MESSERSCHMIDT, Bernhard SCHINK, Peter M. H. KRONECK, 2002. Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici. In: Acta Crystallographica. 2002, 58(2), pp. 343-345. ISSN 0907-4449. eISSN 1399-0047eng
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