Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici

Abt, Dietmar J.; Einsle, Oliver; Niessen, Holger; Krieger, Robert; Messerschmidt, Albrecht; Schink, Bernhard; Kroneck, Peter M. H.

Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici

Dateien

Crystallization_and_preliminary_X_ray_analysis_of_the_molybdenum_dependent_pyrogallol_phloroglucinol_transhydroxylase_of_Pelobacter_acidigallici.pdfGröße: 284.37 KBDownloads: 510

Datum

2002

Autor:innen

Abt, Dietmar J.

Einsle, Oliver

Niessen, Holger

Krieger, Robert

Messerschmidt, Albrecht

Schink, Bernhard

Kroneck, Peter M. H.

URI (zitierfähiger Link)

http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-59892

Link zur Lizenz

Urheberrechtlich geschützt

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Acta Crystallographica. 2002, 58(2), pp. 343-345. ISSN 0907-4449. eISSN 1399-0047

Zusammenfassung

Crystals of the molybdo-/iron±sulfur protein pyrogallol:phloroglucinol hydroxyltransferase (transhydroxylase; EC 1.97.1.2) from Pelobacter acidigallici were grown by vapour diffusion in an N2/H2 atmosphere using polyethylene glycol as a precipitant. In this microorganism, transhydroxylase converts pyrogallol to phloroglucinol in a unique reaction without oxygen transfer from water.
Growth of crystals suitable for X-ray analysis was strongly dependent on the presence of dithionite as a reducing agent. The crystals belonged to space group P1 and MAD data were collected on the iron K edge to resolutions higher than 2.5 .

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

ABT, Dietmar J., Oliver EINSLE, Holger NIESSEN, Robert KRIEGER, Albrecht MESSERSCHMIDT, Bernhard SCHINK, Peter M. H. KRONECK, 2002. Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici. In: Acta Crystallographica. 2002, 58(2), pp. 343-345. ISSN 0907-4449. eISSN 1399-0047

BibTex

@article{Abt2002Cryst-7317,
  year={2002},
  title={Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici},
  number={2},
  volume={58},
  issn={0907-4449},
  journal={Acta Crystallographica},
  pages={343--345},
  author={Abt, Dietmar J. and Einsle, Oliver and Niessen, Holger and Krieger, Robert and Messerschmidt, Albrecht and Schink, Bernhard and Kroneck, Peter M. H.}
}

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Universitätsbibliographie

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