Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici

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2002
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Abt, Dietmar J.
Einsle, Oliver
Niessen, Holger
Krieger, Robert
Messerschmidt, Albrecht
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Acta Crystallographica. 2002, 58(2), pp. 343-345. ISSN 0907-4449. eISSN 1399-0047
Zusammenfassung

Crystals of the molybdo-/iron±sulfur protein pyrogallol:phloroglucinol hydroxyltransferase (transhydroxylase; EC 1.97.1.2) from Pelobacter acidigallici were grown by vapour diffusion in an N2/H2 atmosphere using polyethylene glycol as a precipitant. In this microorganism, transhydroxylase converts pyrogallol to phloroglucinol in a unique reaction without oxygen transfer from water.
Growth of crystals suitable for X-ray analysis was strongly dependent on the presence of dithionite as a reducing agent. The crystals belonged to space group P1 and MAD data were collected on the iron K edge to resolutions higher than 2.5 .

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570 Biowissenschaften, Biologie
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ISO 690ABT, Dietmar J., Oliver EINSLE, Holger NIESSEN, Robert KRIEGER, Albrecht MESSERSCHMIDT, Bernhard SCHINK, Peter M. H. KRONECK, 2002. Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici. In: Acta Crystallographica. 2002, 58(2), pp. 343-345. ISSN 0907-4449. eISSN 1399-0047
BibTex
@article{Abt2002Cryst-7317,
  year={2002},
  title={Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici},
  number={2},
  volume={58},
  issn={0907-4449},
  journal={Acta Crystallographica},
  pages={343--345},
  author={Abt, Dietmar J. and Einsle, Oliver and Niessen, Holger and Krieger, Robert and Messerschmidt, Albrecht and Schink, Bernhard and Kroneck, Peter M. H.}
}
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