Quenched flow analysis of exocytosis in Paramecium cells : time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells

Kurzanzeige
dc.contributor.authorKnoll, Gerddeu
dc.contributor.authorBraun, Claudiadeu
dc.contributor.authorPlattner, Helmut
dc.date.accessioned2011-03-24T17:34:22Zdeu
dc.date.available2011-03-24T17:34:22Zdeu
dc.date.issued1991deu
dc.description.abstractSynchronous exocytosis in Paramecium cells was analyzed on a subsecond time scale. For this purpose we developed a quenched flow device for rapid mixing and rapid freezing of cells without impairment (time resolution in the millisecond range, dead time ~30 ms). Cells frozen at defined times after stimulation with the noncytotoxic secretagogue aminoethyldextran were processed by freeze substitution for electron microscopic analysis. With ultrathin sections the time required for complete extrusion of secretory contents was determined to beeng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Journal of Cell Biology 113 (1991), 6, pp. 1295-1304deu
dc.identifier.ppn274693984deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/7429
dc.language.isodeudeu
dc.legacy.dateIssued2007deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subject.ddc570deu
dc.titleQuenched flow analysis of exocytosis in Paramecium cells : time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cellsdeu
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Knoll1991Quenc-7429,
  year={1991},
  title={Quenched flow analysis of exocytosis in Paramecium cells : time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells},
  number={6},
  volume={113},
  journal={Journal of Cell Biology},
  pages={1295--1304},
  author={Knoll, Gerd and Braun, Claudia and Plattner, Helmut}
}
kops.citation.iso690KNOLL, Gerd, Claudia BRAUN, Helmut PLATTNER, 1991. Quenched flow analysis of exocytosis in Paramecium cells : time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells. In: Journal of Cell Biology. 1991, 113(6), pp. 1295-1304deu
kops.citation.iso690KNOLL, Gerd, Claudia BRAUN, Helmut PLATTNER, 1991. Quenched flow analysis of exocytosis in Paramecium cells : time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells. In: Journal of Cell Biology. 1991, 113(6), pp. 1295-1304eng
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kops.sourcefield.plainJournal of Cell Biology. 1991, 113(6), pp. 1295-1304eng
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