Quenched flow analysis of exocytosis in Paramecium cells : time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells

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1991
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Knoll, Gerd
Braun, Claudia
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Synchronous exocytosis in Paramecium cells was analyzed on a subsecond time scale. For this purpose we developed a quenched flow device for rapid mixing and rapid freezing of cells without impairment (time resolution in the millisecond range, dead time ~30 ms). Cells frozen at defined times after stimulation with the noncytotoxic secretagogue aminoethyldextran were processed by freeze substitution for electron microscopic analysis. With ultrathin sections the time required for complete extrusion of secretory contents was determined to be

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ISO 690KNOLL, Gerd, Claudia BRAUN, Helmut PLATTNER, 1991. Quenched flow analysis of exocytosis in Paramecium cells : time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells. In: Journal of Cell Biology. 1991, 113(6), pp. 1295-1304
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@article{Knoll1991Quenc-7429,
  year={1991},
  title={Quenched flow analysis of exocytosis in Paramecium cells : time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells},
  number={6},
  volume={113},
  journal={Journal of Cell Biology},
  pages={1295--1304},
  author={Knoll, Gerd and Braun, Claudia and Plattner, Helmut}
}
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