Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase

Kurzanzeige
dc.contributor.authorPollegioni, Loredanodeu
dc.contributor.authorHarris, Christopher M.deu
dc.contributor.authorMolla, Gianlucadeu
dc.contributor.authorPilone, Mirella S.deu
dc.contributor.authorGhisla, Sandro
dc.date.accessioned2011-03-24T17:31:14Zdeu
dc.date.available2011-03-24T17:31:14Zdeu
dc.date.issued2001deu
dc.description.abstractD-Amino acid oxidase (DAAO) is a flavoprotein oxidase that catalyzes the oxidation of amino acids and produces ketoacids and H2O2. The rate of product release from reduced DAAO from Rhodotorula gracilis is pH dependent and reflects a pKa of ~9.3. Binding of benzoate and 3,3,3-trifluoro-Image-alanine to wild-type and Y238F DAAO is also pH dependent (pKa=9.8±0.1 and 9.05±0.1, respectively for benzoate binding). However, binding of benzoate to Y223F DAAO is pH independent, indicating the pKa is due to Y223 OH. This latter residue is thus involved in substrate binding, and probably is the group that governs product release. In contrast to this, the second active site tyrosine, Y238, has little influence on ligand binding.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: FEBS letters 507 (2001), 3, pp. 323-326deu
dc.identifier.doi10.1016/S0014-5793(01)02983-0
dc.identifier.ppn278390528deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/7065
dc.language.isoengdeu
dc.legacy.dateIssued2008deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subjectD-Amino acid oxidasedeu
dc.subjectFlavoproteindeu
dc.subjectpH effectdeu
dc.subjectLigand bindingdeu
dc.subjectIonizationdeu
dc.subjectCatalytic mechanismdeu
dc.subject.ddc570deu
dc.titleIdentification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidaseeng
dc.typeJOURNAL_ARTICLEdeu
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@article{Pollegioni2001Ident-7065,
  year={2001},
  doi={10.1016/S0014-5793(01)02983-0},
  title={Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase},
  number={3},
  volume={507},
  issn={0014-5793},
  journal={FEBS letters},
  pages={323--326},
  author={Pollegioni, Loredano and Harris, Christopher M. and Molla, Gianluca and Pilone, Mirella S. and Ghisla, Sandro}
}
kops.citation.iso690POLLEGIONI, Loredano, Christopher M. HARRIS, Gianluca MOLLA, Mirella S. PILONE, Sandro GHISLA, 2001. Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase. In: FEBS letters. 2001, 507(3), pp. 323-326. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(01)02983-0deu
kops.citation.iso690POLLEGIONI, Loredano, Christopher M. HARRIS, Gianluca MOLLA, Mirella S. PILONE, Sandro GHISLA, 2001. Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase. In: FEBS letters. 2001, 507(3), pp. 323-326. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(01)02983-0eng
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