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Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase

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Identification_and_role_of_ionizing_functional_groups_at_the_active_center_of_Rhodotorula_gracilis_D_amino_acid_oxidase.pdf
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Datum

2001

Autor:innen

Pollegioni, Loredano
Harris, Christopher M.
Molla, Gianluca
Pilone, Mirella S.

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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-51553
DOI (zitierfähiger Link)
https://doi.org/10.1016/S0014-5793(01)02983-0
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Attribution-NonCommercial-NoDerivs 2.0 Generic

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Open Access Green

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Biologie: Publikationen
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Published

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FEBS letters. 2001, 507(3), pp. 323-326. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(01)02983-0

Zusammenfassung

D-Amino acid oxidase (DAAO) is a flavoprotein oxidase that catalyzes the oxidation of amino acids and produces ketoacids and H2O2. The rate of product release from reduced DAAO from Rhodotorula gracilis is pH dependent and reflects a pKa of ~9.3. Binding of benzoate and 3,3,3-trifluoro-Image-alanine to wild-type and Y238F DAAO is also pH dependent (pKa=9.8±0.1 and 9.05±0.1, respectively for benzoate binding). However, binding of benzoate to Y223F DAAO is pH independent, indicating the pKa is due to Y223 OH. This latter residue is thus involved in substrate binding, and probably is the group that governs product release. In contrast to this, the second active site tyrosine, Y238, has little influence on ligand binding.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

D-Amino acid oxidase, Flavoprotein, pH effect, Ligand binding, Ionization, Catalytic mechanism

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ISO 690POLLEGIONI, Loredano, Christopher M. HARRIS, Gianluca MOLLA, Mirella S. PILONE, Sandro GHISLA, 2001. Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase. In: FEBS letters. 2001, 507(3), pp. 323-326. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(01)02983-0
BibTex
@article{Pollegioni2001Ident-7065,
  year={2001},
  doi={10.1016/S0014-5793(01)02983-0},
  title={Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase},
  number={3},
  volume={507},
  issn={0014-5793},
  journal={FEBS letters},
  pages={323--326},
  author={Pollegioni, Loredano and Harris, Christopher M. and Molla, Gianluca and Pilone, Mirella S. and Ghisla, Sandro}
}
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