Prickly postglacial pioneers : freshwater plankton community composition influences fatty acid desaturase (FADS2) copy number in Southern Greenland threespine sticklebacks

dc.contributor.authorHudson, Cameron
dc.contributor.authorTwining, Cornelia W.
dc.contributor.authorMoosmann, Marvin
dc.contributor.authorGreenway, Ryan
dc.contributor.authorFeulner, Philine
dc.contributor.authorMatthews, Blake
dc.date.accessioned2025-01-22T11:08:02Z
dc.date.available2025-01-22T11:08:02Z
dc.date.created2024-10-07T15:56:14.000Z
dc.date.issued2024
dc.description.abstractThe adaptation of marine fish to freshwater environments includes prodigious examples of rapid evolution. Given the scarcity of ω-3 long-chain polyunsaturated fatty acids (LC-PUFA) in freshwater, we expect selection to be strong on fish transitioning to freshwater habitats and yet the underlying ecological causes of genomic and phenotypic differentiation are poorly understood for traits associated with lipid content and composition. Threespine stickleback (Gasterosteus aculeatus) have repeatedly colonized, and adapted to, freshwater habitats across the northern hemisphere. These freshwater populations often show elevated copy number of the fatty acid desaturase 2 gene (FADS2), which increases the biosynthetic capacity of LC-PUFA. The starkly lower content of LC-PUFA in freshwater compared to marine prey, especially docosahexaenoic acid (DHA), likely imposes strong positive selection on freshwater fish for either increased biosynthesis or greater dietary acquisition of LC-PUFA. The recently colonized and relatively undisturbed threespine stickleback populations in postglacial coastal lakes of Southern Greenland offer an exceptional opportunity to study how variation in the copy number of FADS2 is related to abiotic and biotic conditions of lakes and their morphometry. As expected, given its position on the stickleback X chromosome, we found strong sexual dimorphism in FADS2 copy number in all populations (19 freshwater, 1 marine, and 1 brackish), and an increased dimorphism in some freshwater populations. We also found that FADS2 copy number was negatively correlated, for both males and females, with the abundance of copepods, which are a DHA-rich food source in the zooplankton community. Overall, our results suggest that the prey community context of lakes might influence the process of metabolic adaptation of marine fish colonizing freshwater ecosystems.
dc.description.versionpublished
dc.identifier.doi10.5061/dryad.z08kprrpm
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/72005
dc.language.isoeng
dc.relation.hasparthttps://doi.org/10.5281/zenodo.13790206
dc.relation.hasparthttps://doi.org/10.5281/zenodo.13790210
dc.relation.isreferencedbyhttps://doi.org/10.22541/au.172455403.33805173/v1
dc.rightsCreative Commons Zero v1.0 Universal
dc.rights.urihttps://creativecommons.org/publicdomain/zero/1.0/legalcode
dc.subjectadaptation
dc.subjectColonization
dc.subjectFADS2
dc.subjectLong-chain polyunsaturated fatty acids
dc.subjectthreespine stickleback
dc.subjectCopy number variation
dc.subjectFOS: Biological sciences
dc.subject.ddc570
dc.titlePrickly postglacial pioneers : freshwater plankton community composition influences fatty acid desaturase (FADS2) copy number in Southern Greenland threespine sticklebackseng
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kops.citation.iso690HUDSON, Cameron, Cornelia W. TWINING, Marvin MOOSMANN, Ryan GREENWAY, Philine FEULNER, Blake MATTHEWS, 2024. Prickly postglacial pioneers : freshwater plankton community composition influences fatty acid desaturase (FADS2) copy number in Southern Greenland threespine sticklebacksdeu
kops.citation.iso690HUDSON, Cameron, Cornelia W. TWINING, Marvin MOOSMANN, Ryan GREENWAY, Philine FEULNER, Blake MATTHEWS, 2024. Prickly postglacial pioneers : freshwater plankton community composition influences fatty acid desaturase (FADS2) copy number in Southern Greenland threespine sticklebackseng
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