Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma

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2024
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Schultze-Rhonhof, Laura
Marzi, Julia
Carvajal Berrio, Daniel Alejandro
Holl, Myriam
Schäfer-Ruoff, Felix
Andress, Jürgen
Bachmann, Cornelia
Templin, Markus
Weiss, Martin
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Frontiers in Immunology. Frontiers Media. 2024, 15, 1357340. eISSN 1664-3224. Verfügbar unter: doi: 10.3389/fimmu.2024.1357340
Zusammenfassung

In the context of multimodal treatments for abdominal cancer, including procedures such as cytoreductive surgery and intraperitoneal chemotherapy, recurrence rates remain high, and long-term survival benefits are uncertain due to post-operative complications. Notably, treatment-limiting side effects often arise from an uncontrolled activation of the immune system, particularly peritoneally localized macrophages, leading to massive cytokine secretion and phenotype changes. Exploring alternatives, an increasing number of studies investigated the potential of plasma-activated liquids (PAL) for adjuvant peritoneal cancer treatment, aiming to mitigate side effects, preserve healthy tissue, and reduce cytotoxicity towards non-cancer cells. To assess the non-toxicity of PAL, we isolated primary human macrophages from the peritoneum and subjected them to PAL exposure. Employing an extensive methodological spectrum, including flow cytometry, Raman microspectroscopy, and DigiWest protein analysis, we observed a pronounced resistance of macrophages towards PAL. This resistance was characterized by an upregulation of proliferation and anti-oxidative pathways, countering PAL-derived oxidative stress-induced cell death. The observed cellular effects of PAL treatment on human tissue-resident peritoneal macrophages unveil a potential avenue for PAL-derived immunomodulatory effects within the human peritoneal cavity. Our findings contribute to understanding the intricate interplay between PAL and macrophages, shedding light on the promising prospects for PAL in the adjuvant treatment of peritoneal cancer.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
non-invasive physical plasma (NIPP), cold atmospheric plasma (CAP), plasma-activated media (PAM), plasma-treated solutions (PTS), human primary macrophages, immune response, peritoneal cavity, peritoneal cancer
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ISO 690SCHULTZE-RHONHOF, Laura, Julia MARZI, Daniel Alejandro CARVAJAL BERRIO, Myriam HOLL, Theresa S. BRAUN, Felix SCHÄFER-RUOFF, Jürgen ANDRESS, Cornelia BACHMANN, Markus TEMPLIN, Martin WEISS, 2024. Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma. In: Frontiers in Immunology. Frontiers Media. 2024, 15, 1357340. eISSN 1664-3224. Verfügbar unter: doi: 10.3389/fimmu.2024.1357340
BibTex
@article{SchultzeRhonhof2024-03-05Human-69941,
  year={2024},
  doi={10.3389/fimmu.2024.1357340},
  title={Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma},
  volume={15},
  journal={Frontiers in Immunology},
  author={Schultze-Rhonhof, Laura and Marzi, Julia and Carvajal Berrio, Daniel Alejandro and Holl, Myriam and Braun, Theresa S. and Schäfer-Ruoff, Felix and Andress, Jürgen and Bachmann, Cornelia and Templin, Markus and Weiss, Martin},
  note={Article Number: 1357340}
}
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