Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking

Lade...
Vorschaubild
Dateien
Vogel_2-ore8xb9nto0y5.PDF
Vogel_2-ore8xb9nto0y5.PDFGröße: 7.9 MBDownloads: 64
Datum
2022
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Gold
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Nature communications. Springer Nature. 2022, 13(1), 6897. eISSN 2041-1723. Available under: doi: 10.1038/s41467-022-34637-3
Zusammenfassung

The abundance of plasma membrane-resident receptors and transporters has to be tightly regulated by ubiquitin-mediated endosomal degradation for the proper coordination of environmental stimuli and intracellular signaling. Arabidopsis OVARIAN TUMOR PROTEASE (OTU) 11 and OTU12 are plasma membrane-localized deubiquitylating enzymes (DUBs) that bind to phospholipids through a polybasic motif in the OTU domain. Here we show that the DUB activity of OTU11 and OTU12 towards K63-linked ubiquitin is stimulated by binding to lipid membranes containing anionic lipids. In addition, we show that the DUB activity of OTU11 against K6- and K11-linkages is also stimulated by anionic lipids, and that OTU11 and OTU12 can modulate the endosomal degradation of a model cargo and the auxin efflux transporter PIN2-GFP in vivo. Our results suggest that the catalytic activity of OTU11 and OTU12 is tightly connected to their ability to bind membranes and that OTU11 and OTU12 are involved in the fine-tuning of plasma membrane proteins in Arabidopsis.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Confocal microscopy, Post-translational modifications, Proteases, Protein trafficking in plants
Konferenz
Rezension
undefined / . - undefined, undefined
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Datensätze
Zitieren
ISO 690VOGEL, Karin, Tobias BLÄSKE, Marie-Kristin NAGEL, Christoph GLOBISCH, Shane MAGUIRE, Lorenz MATTES, Michael KOVERMANN, Karin HAUSER, Christine PETER, Erika ISONO, 2022. Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking. In: Nature communications. Springer Nature. 2022, 13(1), 6897. eISSN 2041-1723. Available under: doi: 10.1038/s41467-022-34637-3
BibTex
@article{Vogel2022-11-12Lipid-59176,
  year={2022},
  doi={10.1038/s41467-022-34637-3},
  title={Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking},
  number={1},
  volume={13},
  journal={Nature communications},
  author={Vogel, Karin and Bläske, Tobias and Nagel, Marie-Kristin and Globisch, Christoph and Maguire, Shane and Mattes, Lorenz and Kovermann, Michael and Hauser, Karin and Peter, Christine and Isono, Erika},
  note={Article Number: 6897}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/59176">
    <dc:creator>Isono, Erika</dc:creator>
    <dc:contributor>Mattes, Lorenz</dc:contributor>
    <dc:contributor>Bläske, Tobias</dc:contributor>
    <dc:creator>Globisch, Christoph</dc:creator>
    <dc:creator>Kovermann, Michael</dc:creator>
    <dcterms:title>Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal trafficking</dcterms:title>
    <dc:creator>Peter, Christine</dc:creator>
    <dc:creator>Maguire, Shane</dc:creator>
    <dc:creator>Bläske, Tobias</dc:creator>
    <dc:creator>Hauser, Karin</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-17T09:45:23Z</dc:date>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dc:contributor>Hauser, Karin</dc:contributor>
    <dc:contributor>Maguire, Shane</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/59176"/>
    <dc:contributor>Isono, Erika</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:contributor>Globisch, Christoph</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:contributor>Peter, Christine</dc:contributor>
    <dc:language>eng</dc:language>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/59176/1/Vogel_2-ore8xb9nto0y5.PDF"/>
    <dc:creator>Vogel, Karin</dc:creator>
    <dc:contributor>Kovermann, Michael</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/59176/1/Vogel_2-ore8xb9nto0y5.PDF"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>2022-11-12</dcterms:issued>
    <dc:contributor>Vogel, Karin</dc:contributor>
    <dc:creator>Mattes, Lorenz</dc:creator>
    <dc:creator>Nagel, Marie-Kristin</dc:creator>
    <dcterms:abstract xml:lang="eng">The abundance of plasma membrane-resident receptors and transporters has to be tightly regulated by ubiquitin-mediated endosomal degradation for the proper coordination of environmental stimuli and intracellular signaling. Arabidopsis OVARIAN TUMOR PROTEASE (OTU) 11 and OTU12 are plasma membrane-localized deubiquitylating enzymes (DUBs) that bind to phospholipids through a polybasic motif in the OTU domain. Here we show that the DUB activity of OTU11 and OTU12 towards K63-linked ubiquitin is stimulated by binding to lipid membranes containing anionic lipids. In addition, we show that the DUB activity of OTU11 against K6- and K11-linkages is also stimulated by anionic lipids, and that OTU11 and OTU12 can modulate the endosomal degradation of a model cargo and the auxin efflux transporter PIN2-GFP in vivo. Our results suggest that the catalytic activity of OTU11 and OTU12 is tightly connected to their ability to bind membranes and that OTU11 and OTU12 are involved in the fine-tuning of plasma membrane proteins in Arabidopsis.</dcterms:abstract>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Nagel, Marie-Kristin</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-17T09:45:23Z</dcterms:available>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen