KOPS - The Institutional Repository of the University of Konstanz

Conformational States Control Lck Switching between Free and Confined Diffusion Modes in T Cells

Conformational States Control Lck Switching between Free and Confined Diffusion Modes in T Cells

Cite This

Files in this item

Checksum: MD5:9b79714156f12225108b30627f1932ca

HILZENRAT, Geva, Elvis PANDŽIĆ, Zhengmin YANG, Daniel J. NIEVES, Jesse GOYETTE, Jérémie ROSSY, Yuanqing MA, Katharina GAUS, 2020. Conformational States Control Lck Switching between Free and Confined Diffusion Modes in T Cells. In: Biophysical journal. Elsevier. 118(6), pp. 1489-1501. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2020.01.041

@article{Hilzenrat2020Confo-58132, title={Conformational States Control Lck Switching between Free and Confined Diffusion Modes in T Cells}, year={2020}, doi={10.1016/j.bpj.2020.01.041}, number={6}, volume={118}, issn={0006-3495}, journal={Biophysical journal}, pages={1489--1501}, author={Hilzenrat, Geva and Pandžić, Elvis and Yang, Zhengmin and Nieves, Daniel J. and Goyette, Jesse and Rossy, Jérémie and Ma, Yuanqing and Gaus, Katharina} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/58132"> <dc:rights>terms-of-use</dc:rights> <dcterms:title>Conformational States Control Lck Switching between Free and Confined Diffusion Modes in T Cells</dcterms:title> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/58132/1/Hilzenrat_2-9hakr0qa2uef0.pdf"/> <dc:creator>Goyette, Jesse</dc:creator> <dc:creator>Pandžić, Elvis</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/58132/1/Hilzenrat_2-9hakr0qa2uef0.pdf"/> <dc:creator>Ma, Yuanqing</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/58132"/> <dc:contributor>Yang, Zhengmin</dc:contributor> <dc:contributor>Pandžić, Elvis</dc:contributor> <dc:contributor>Gaus, Katharina</dc:contributor> <dc:creator>Hilzenrat, Geva</dc:creator> <dc:contributor>Rossy, Jérémie</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Nieves, Daniel J.</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-07-22T08:37:05Z</dcterms:available> <dc:creator>Rossy, Jérémie</dc:creator> <dc:contributor>Hilzenrat, Geva</dc:contributor> <dc:creator>Nieves, Daniel J.</dc:creator> <dc:contributor>Ma, Yuanqing</dc:contributor> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-07-22T08:37:05Z</dc:date> <dcterms:issued>2020</dcterms:issued> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Gaus, Katharina</dc:creator> <dcterms:abstract xml:lang="eng">T cell receptor phosphorylation by Lck is an essential step in T cell activation. It is known that the conformational states of Lck control enzymatic activity; however, the underlying principles of how Lck finds its substrate over the plasma membrane remain elusive. Here, single-particle tracking is paired with photoactivatable localization microscopy to observe the diffusive modes of Lck in the plasma membrane. Individual Lck molecules switched between free and confined diffusion in both resting and stimulated T cells. Lck mutants locked in the open conformation were more confined than Lck mutants in the closed conformation. Further confinement of kinase-dead versions of Lck suggests that Lck confinement was not caused by phosphorylated substrates. Our data support a model in which confined diffusion of open Lck results in high local phosphorylation rates, and inactive, closed Lck diffuses freely to enable long-range distribution over the plasma membrane.</dcterms:abstract> <dc:creator>Yang, Zhengmin</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Goyette, Jesse</dc:contributor> </rdf:Description> </rdf:RDF>

Downloads since Jul 22, 2022 (Information about access statistics)

Hilzenrat_2-9hakr0qa2uef0.pdf 15

This item appears in the following Collection(s)

Search KOPS


Browse

My Account