A topological refactoring design strategy yields highly stable granulopoietic proteins

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2022
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Skokowa, Julia
Hernandez Alvarez, Birte
Coles, Murray
Ritter, Malte
Nasri, Masoud
Haaf, Jérémy
Aghaallaei, Narges
Xu, Yun
Mir, Perihan
Krahl, Ann-Christin
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Nature communications. Nature Publishing Group. 2022, 13(1), 2948. eISSN 2041-1723. Available under: doi: 10.1038/s41467-022-30157-2
Zusammenfassung

Protein therapeutics frequently face major challenges, including complicated production, instability, poor solubility, and aggregation. De novo protein design can readily address these challenges. Here, we demonstrate the utility of a topological refactoring strategy to design novel granulopoietic proteins starting from the granulocyte-colony stimulating factor (G-CSF) structure. We change a protein fold by rearranging the sequence and optimising it towards the new fold. Testing four designs, we obtain two that possess nanomolar activity, the most active of which is highly thermostable and protease-resistant, and matches its designed structure to atomic accuracy. While the designs possess starkly different sequence and structure from the native G-CSF, they show specific activity in differentiating primary human haematopoietic stem cells into mature neutrophils. The designs also show significant and specific activity in vivo. Our topological refactoring approach is largely independent of sequence or structural context, and is therefore applicable to a wide range of protein targets.

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ISO 690SKOKOWA, Julia, Birte HERNANDEZ ALVAREZ, Murray COLES, Malte RITTER, Masoud NASRI, Jérémy HAAF, Narges AGHAALLAEI, Yun XU, Perihan MIR, Ann-Christin KRAHL, Patrick MÜLLER, 2022. A topological refactoring design strategy yields highly stable granulopoietic proteins. In: Nature communications. Nature Publishing Group. 2022, 13(1), 2948. eISSN 2041-1723. Available under: doi: 10.1038/s41467-022-30157-2
BibTex
@article{Skokowa2022-05-26topol-57761,
  year={2022},
  doi={10.1038/s41467-022-30157-2},
  title={A topological refactoring design strategy yields highly stable granulopoietic proteins},
  number={1},
  volume={13},
  journal={Nature communications},
  author={Skokowa, Julia and Hernandez Alvarez, Birte and Coles, Murray and Ritter, Malte and Nasri, Masoud and Haaf, Jérémy and Aghaallaei, Narges and Xu, Yun and Mir, Perihan and Krahl, Ann-Christin and Müller, Patrick},
  note={Article Number: 2948}
}
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