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Mechanism of signal sequence handover from NAC to SRP on ribosomes during ER-protein targeting

Mechanism of signal sequence handover from NAC to SRP on ribosomes during ER-protein targeting

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JOMAA, Ahmad, Martin GAMERDINGER, Hao-Hsuan HSIEH, Annalena WALLISCH, Viswanathan CHANDRASEKARAN, Zeynel ULUSOY, Alain SCAIOLA, Shu-ou SHAN, Nenad BAN, Elke DEUERLING, 2022. Mechanism of signal sequence handover from NAC to SRP on ribosomes during ER-protein targeting. In: Science. American Association for the Advancement of Science (AAAS). 375(6583), pp. 839-844. ISSN 0036-8075. eISSN 1095-9203. Available under: doi: 10.1126/science.abl6459

@article{Jomaa2022Mecha-57095, title={Mechanism of signal sequence handover from NAC to SRP on ribosomes during ER-protein targeting}, year={2022}, doi={10.1126/science.abl6459}, number={6583}, volume={375}, issn={0036-8075}, journal={Science}, pages={839--844}, author={Jomaa, Ahmad and Gamerdinger, Martin and Hsieh, Hao-Hsuan and Wallisch, Annalena and Chandrasekaran, Viswanathan and Ulusoy, Zeynel and Scaiola, Alain and Shan, Shu-ou and Ban, Nenad and Deuerling, Elke} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/57095"> <dc:creator>Deuerling, Elke</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-30T12:32:25Z</dcterms:available> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Scaiola, Alain</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Shan, Shu-ou</dc:contributor> <dc:contributor>Gamerdinger, Martin</dc:contributor> <dc:creator>Hsieh, Hao-Hsuan</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Wallisch, Annalena</dc:creator> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:creator>Shan, Shu-ou</dc:creator> <dc:creator>Chandrasekaran, Viswanathan</dc:creator> <dc:contributor>Wallisch, Annalena</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-30T12:32:25Z</dc:date> <dc:rights>terms-of-use</dc:rights> <dcterms:abstract xml:lang="eng">The nascent polypeptide-associated complex (NAC) interacts with newly synthesized proteins at the ribosomal tunnel exit and competes with the signal recognition particle (SRP) to prevent mistargeting of cytosolic and mitochondrial polypeptides to the endoplasmic reticulum (ER). How NAC antagonizes SRP and how this is overcome by ER targeting signals are unknown. Here, we found that NAC uses two domains with opposing effects to control SRP access. The core globular domain prevented SRP from binding to signal-less ribosomes, whereas a flexibly attached domain transiently captured SRP to permit scanning of nascent chains. The emergence of an ER-targeting signal destabilized NAC's globular domain and facilitated SRP access to the nascent chain. These findings elucidate how NAC hands over the signal sequence to SRP and imparts specificity of protein localization.</dcterms:abstract> <dc:contributor>Hsieh, Hao-Hsuan</dc:contributor> <dc:creator>Gamerdinger, Martin</dc:creator> <dc:contributor>Chandrasekaran, Viswanathan</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/57095"/> <dc:contributor>Scaiola, Alain</dc:contributor> <dc:contributor>Ulusoy, Zeynel</dc:contributor> <dc:creator>Ban, Nenad</dc:creator> <dc:contributor>Deuerling, Elke</dc:contributor> <dc:creator>Jomaa, Ahmad</dc:creator> <dc:language>eng</dc:language> <dc:creator>Ulusoy, Zeynel</dc:creator> <dc:contributor>Ban, Nenad</dc:contributor> <dc:contributor>Jomaa, Ahmad</dc:contributor> <dcterms:issued>2022</dcterms:issued> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:title>Mechanism of signal sequence handover from NAC to SRP on ribosomes during ER-protein targeting</dcterms:title> </rdf:Description> </rdf:RDF>

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