KOPS - The Institutional Repository of the University of Konstanz

SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling

SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling

Cite This

Files in this item

Checksum: MD5:08897935cd2eb4aa6d6738089173cdf6

ECKER, Manuela, Richard SCHREGLE, Natasha KAPOOR-KAUSHIK, Pascal ROSSATTI, Verena M. BETZLER, Daryan KEMPE, Maté BIRO, Nicholas ARIOTTI, Gregory Mi REDPATH, Jérémie ROSSY, 2022. SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling. In: eLife. eLife Sciences Publications. 11, e67550. eISSN 2050-084X. Available under: doi: 10.7554/eLife.67550

@article{Ecker2022-01-20SNX9i-56518, title={SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling}, year={2022}, doi={10.7554/eLife.67550}, volume={11}, journal={eLife}, author={Ecker, Manuela and Schregle, Richard and Kapoor-Kaushik, Natasha and Rossatti, Pascal and Betzler, Verena M. and Kempe, Daryan and Biro, Maté and Ariotti, Nicholas and Redpath, Gregory Mi and Rossy, Jérémie}, note={Article Number: e67550} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/56518"> <dc:contributor>Redpath, Gregory Mi</dc:contributor> <dc:creator>Ecker, Manuela</dc:creator> <dc:contributor>Ecker, Manuela</dc:contributor> <dc:contributor>Biro, Maté</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Ariotti, Nicholas</dc:creator> <dc:creator>Betzler, Verena M.</dc:creator> <dc:rights>terms-of-use</dc:rights> <dcterms:title>SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling</dcterms:title> <dc:creator>Schregle, Richard</dc:creator> <dc:creator>Rossatti, Pascal</dc:creator> <dcterms:issued>2022-01-20</dcterms:issued> <dc:contributor>Ariotti, Nicholas</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Kapoor-Kaushik, Natasha</dc:contributor> <dc:creator>Redpath, Gregory Mi</dc:creator> <dcterms:abstract xml:lang="eng">T cell activation requires engagement of a cognate antigen by the T cell receptor (TCR) and the co-stimulatory signal of CD28. Both TCR and CD28 aggregate into clusters at the plasma membrane of activated T cells. While the role of TCR clustering in T cell activation has been extensively investigated, little is known about how CD28 clustering contributes to CD28 signalling. Here, we report that upon CD28 triggering, the BAR-domain protein sorting nexin 9 (SNX9) is recruited to CD28 clusters at the immunological synapse. Using three-dimensional correlative light and electron microscopy, we show that SNX9 generates membrane tubulation out of CD28 clusters. Our data further reveal that CD28 clusters are in fact dynamic structures and that SNX9 regulates their stability as well as CD28 phosphorylation and the resulting production of the cytokine IL-2. In summary, our work suggests a model in which SNX9-mediated tubulation generates a membrane environment that promotes CD28 triggering and downstream signalling events.</dcterms:abstract> <dc:creator>Kempe, Daryan</dc:creator> <dc:contributor>Kempe, Daryan</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Betzler, Verena M.</dc:contributor> <dc:contributor>Schregle, Richard</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/56518"/> <dc:creator>Kapoor-Kaushik, Natasha</dc:creator> <dc:creator>Biro, Maté</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-02-10T13:19:53Z</dcterms:available> <dc:contributor>Rossy, Jérémie</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56518/1/Ecker_2-12p2dagciavlh1.pdf"/> <dc:creator>Rossy, Jérémie</dc:creator> <dc:contributor>Rossatti, Pascal</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-02-10T13:19:53Z</dc:date> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56518/1/Ecker_2-12p2dagciavlh1.pdf"/> </rdf:Description> </rdf:RDF>

Downloads since Feb 10, 2022 (Information about access statistics)

Ecker_2-12p2dagciavlh1.pdf 29

This item appears in the following Collection(s)

Search KOPS


Browse

My Account