FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos

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SOH, Gary H., Patrick MÜLLER, 2018. FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos. In: DUBRULLE, Julien, ed.. Morphogen Gradients : Methods and Protocols. New York:Humana Press, pp. 107-124. ISBN 978-1-4939-8771-9. Available under: doi: 10.1007/978-1-4939-8772-6_6

@incollection{Soh2018Analy-56434, title={FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos}, year={2018}, doi={10.1007/978-1-4939-8772-6_6}, number={1863}, isbn={978-1-4939-8771-9}, address={New York}, publisher={Humana Press}, series={Methods in Molecular Biology}, booktitle={Morphogen Gradients : Methods and Protocols}, pages={107--124}, editor={Dubrulle, Julien}, author={Soh, Gary H. and Müller, Patrick} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:contributor>Soh, Gary H.</dc:contributor> <dcterms:abstract xml:lang="eng">Morphogens are signaling molecules that provide positional information to cells during development. They must move through embryonic tissues in order to coordinate patterning. The rate of a morphogen's movement through a tissue-its effective diffusivity-affects the morphogen's distribution and therefore influences patterning. Fluorescence recovery after photobleaching (FRAP) is a powerful method to measure the effective diffusion of molecules through cells and tissues, and has been successfully employed to examine morphogen mobility and gain important insights into embryogenesis. Here, we provide detailed protocols for FRAP assays in vitro and in living zebrafish embryos, and we explain how to analyze FRAP data using the open-source software PyFRAP to determine effective diffusion coefficients.</dcterms:abstract> <dcterms:available rdf:datatype="">2022-02-07T14:09:36Z</dcterms:available> <dc:rights>terms-of-use</dc:rights> <dcterms:rights rdf:resource=""/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:isPartOfCollection rdf:resource=""/> <dc:contributor>Müller, Patrick</dc:contributor> <dcterms:isPartOf rdf:resource=""/> <bibo:uri rdf:resource=""/> <dc:language>eng</dc:language> <dc:creator>Soh, Gary H.</dc:creator> <dcterms:issued>2018</dcterms:issued> <dcterms:title>FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos</dcterms:title> <dc:creator>Müller, Patrick</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:date rdf:datatype="">2022-02-07T14:09:36Z</dc:date> </rdf:Description> </rdf:RDF>

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