Guidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0)

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Pamies_2-uckgzfe7ugo07.pdf
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2022
Autor:innen
Pamies, David
Coecke, Sandra
Bowe, Gerard
Allen, David G.
Gstraunthaler, Gerhard
Bal-Price, Anna
Pistollato, Francesca
de Vries, Rob B. M.
et al.
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Alternatives to Animal Experimentation : ALTEX. Springer Spektrum. 2022, 39, pp. 30-70. ISSN 1868-596X. eISSN 1868-8551. Available under: doi: 10.14573/altex.2111011
Zusammenfassung

Good Cell and Tissue Culture Practice (GCCP) 2.0 is an updated guidance document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical use in the laboratory to assure the reproducibility of in vitro (cell-based) work. The update in the guidance was essential as cell models have advanced dramatically to more complex culture systems and need more comprehensive quality management to ensure reproducibility and quality derived scientific data. This document describes six main principles to consider when performing cell culture including characterization and maintenance of essential characteristics, quality management, documentation and reporting, safety, education and training, and ethics. The document does not intend to impose detailed procedures but to describe potential quality issues. It is foreseen that the document will require further updates as the science and technologies evolve over time.

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570 Biowissenschaften, Biologie
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ISO 690PAMIES, David, Marcel LEIST, Sandra COECKE, Gerard BOWE, David G. ALLEN, Gerhard GSTRAUNTHALER, Anna BAL-PRICE, Francesca PISTOLLATO, Rob B. M. DE VRIES, Thomas HARTUNG, 2022. Guidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0). In: Alternatives to Animal Experimentation : ALTEX. Springer Spektrum. 2022, 39, pp. 30-70. ISSN 1868-596X. eISSN 1868-8551. Available under: doi: 10.14573/altex.2111011
BibTex
@article{Pamies2022Guida-55914,
  year={2022},
  doi={10.14573/altex.2111011},
  title={Guidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0)},
  volume={39},
  issn={1868-596X},
  journal={Alternatives to Animal Experimentation : ALTEX},
  pages={30--70},
  author={Pamies, David and Leist, Marcel and Coecke, Sandra and Bowe, Gerard and Allen, David G. and Gstraunthaler, Gerhard and Bal-Price, Anna and Pistollato, Francesca and de Vries, Rob B. M. and Hartung, Thomas}
}
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