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Amino Acid Residues Required for Physical and Cooperative Transcriptional Interaction of STAT3 and AP-1 Proteins c-Jun and c-Fos

Amino Acid Residues Required for Physical and Cooperative Transcriptional Interaction of STAT3 and AP-1 Proteins c-Jun and c-Fos

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GINSBERG, Michael, Elmar CZEKO, Patrick MÜLLER, Zhiyong REN, Xiaomin CHEN, James E. DARNELL, 2007. Amino Acid Residues Required for Physical and Cooperative Transcriptional Interaction of STAT3 and AP-1 Proteins c-Jun and c-Fos. In: Molecular and Cellular Biology. American Society for Microbiology (ASM). 27(18), pp. 6300-6308. ISSN 0270-7306. eISSN 1098-5549. Available under: doi: 10.1128/MCB.00613-07

@article{Ginsberg2007-09Amino-55871, title={Amino Acid Residues Required for Physical and Cooperative Transcriptional Interaction of STAT3 and AP-1 Proteins c-Jun and c-Fos}, year={2007}, doi={10.1128/MCB.00613-07}, number={18}, volume={27}, issn={0270-7306}, journal={Molecular and Cellular Biology}, pages={6300--6308}, author={Ginsberg, Michael and Czeko, Elmar and Müller, Patrick and Ren, Zhiyong and Chen, Xiaomin and Darnell, James E.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/55871"> <dc:contributor>Ren, Zhiyong</dc:contributor> <dcterms:title>Amino Acid Residues Required for Physical and Cooperative Transcriptional Interaction of STAT3 and AP-1 Proteins c-Jun and c-Fos</dcterms:title> <dc:creator>Darnell, James E.</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-12-14T09:30:07Z</dcterms:available> <dc:creator>Ginsberg, Michael</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Chen, Xiaomin</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Müller, Patrick</dc:contributor> <dc:contributor>Ginsberg, Michael</dc:contributor> <dc:creator>Müller, Patrick</dc:creator> <dc:contributor>Czeko, Elmar</dc:contributor> <dc:contributor>Darnell, James E.</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-12-14T09:30:07Z</dc:date> <dcterms:issued>2007-09</dcterms:issued> <dc:creator>Ren, Zhiyong</dc:creator> <dc:language>eng</dc:language> <dcterms:abstract xml:lang="eng">Cooperation between STAT3 and c-Jun in driving transcription during transfection of reporter constructs is well established, and both proteins are present on some interleukin-6 (IL-6) STAT3-dependent promoters on chromosomal loci. We report that small interfering RNA knockdown of c-Jun or c-Fos diminishes IL-6 induction of some but not all STAT3-dependent mRNAs. Specific contact sites in STAT3 responsible for interaction of a domain of STAT3 with c-Jun were known. Here we show that the B-zip domain of c-Jun interacts with STAT3 and that c-Jun mutation R261A or R261D near but not in the DNA binding domain blocks in vitro STAT3-c-Jun interaction and decreases costimulation of transcription in transfection assays. Cooperative binding to DNA of tyrosine-phosphorylated STAT3 and both wild-type and R261A mutant c-Jun was observed. Even c-Jun mutant R261D, which on its own did not bind DNA, bound DNA weakly in the presence of STAT3. We conclude that a functional interaction between STAT3 and c-Jun while bound to chromosomal DNA elements exists and is necessary for driving transcription on at least some STAT3 target genes. Identifying such required interactive protein interfaces should be a stimulus to search for compounds that could ultimately inhibit the activity of STAT3 in tumors dependent on persistently active STAT3.</dcterms:abstract> <dc:rights>terms-of-use</dc:rights> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Chen, Xiaomin</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/55871"/> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:creator>Czeko, Elmar</dc:creator> </rdf:Description> </rdf:RDF>

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