Isolation from a fish kill and transcriptomic characterization of Gyrodinium jinhaense off Long Island Sound

Sprecher, Brittany N.; Zhang, Huan; Park, Gihong; Lin, Senjie

Isolation from a fish kill and transcriptomic characterization of Gyrodinium jinhaense off Long Island Sound

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2021

Autor:innen

Sprecher, Brittany N.

Zhang, Huan

Park, Gihong

Lin, Senjie

DOI (zitierfähiger Link)

https://doi.org/10.1016/j.hal.2021.102136

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Harmful Algae. Elsevier. 2021, 110, 102136. ISSN 1568-9883. eISSN 1878-1470. Available under: doi: 10.1016/j.hal.2021.102136

Zusammenfassung

First found in Korean coastal water, the dinoflagellate Gyrodinium jinhaense is a recently established species with unclear global distribution and unexplored genomic characteristics. From a laboratory fish mortality event off Long Island Sound, USA, we isolated a dinoflagellate, and by microscopic and molecular (18S rRNA gene; >99% identical) analyses found that it resembles G. jinhaense, hence named G. jinhaense strain AP17. Towards developing a genetic database for this dinoflagellate, a transcriptome of this species was sequenced using RNA-seq, producing 6 Gbp of data that was assembled into over 70,000 unigenes. The assembled transcriptome GC content was approximately 56% and the total Benchmarking Universal Single-Copy Orthologs for Eukaryota and Alveolata databases were approximately 50% and 57%, respectfully. Genes involved in grazing, energy generation, genome architecture, and protein synthesis, processing, and degradation were highly represented in the transcriptome. Moreover, fragments of polyketide synthase and saxitoxin genes were found but saxitoxins were not detected in high performance liquid chromatography measurements. With the first reported transcriptome for the Gyrodinium genus, this study will serve as a baseline for future Gyrodinium genomics and toxicological studies.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

SPRECHER, Brittany N., Huan ZHANG, Gihong PARK, Senjie LIN, 2021. Isolation from a fish kill and transcriptomic characterization of Gyrodinium jinhaense off Long Island Sound. In: Harmful Algae. Elsevier. 2021, 110, 102136. ISSN 1568-9883. eISSN 1878-1470. Available under: doi: 10.1016/j.hal.2021.102136

BibTex

@article{Sprecher2021Isola-55803,
  year={2021},
  doi={10.1016/j.hal.2021.102136},
  title={Isolation from a fish kill and transcriptomic characterization of Gyrodinium jinhaense off Long Island Sound},
  volume={110},
  issn={1568-9883},
  journal={Harmful Algae},
  author={Sprecher, Brittany N. and Zhang, Huan and Park, Gihong and Lin, Senjie},
  note={Article Number: 102136}
}

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