Controlling Protein Nanocage Assembly with Hydrostatic Pressure

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LE VAY, Kristian, Ben M. CARTER, Daniel W. WATKINS, Tsing-Young Dora TANG, Valeska P. TING, Helmut CÖLFEN, Robert P. RAMBO, Andrew J. SMITH, J. L. Ross ANDERSON, Adam W. PERRIMAN, 2020. Controlling Protein Nanocage Assembly with Hydrostatic Pressure. In: Journal of the American Chemical Society. American Chemical Society (ACS). 142(49), pp. 20640-20650. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/jacs.0c07285

@article{LeVay2020-12-09Contr-52726, title={Controlling Protein Nanocage Assembly with Hydrostatic Pressure}, year={2020}, doi={10.1021/jacs.0c07285}, number={49}, volume={142}, issn={0002-7863}, journal={Journal of the American Chemical Society}, pages={20640--20650}, author={Le Vay, Kristian and Carter, Ben M. and Watkins, Daniel W. and Tang, Tsing-Young Dora and Ting, Valeska P. and Cölfen, Helmut and Rambo, Robert P. and Smith, Andrew J. and Anderson, J. L. Ross and Perriman, Adam W.} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dcterms:available rdf:datatype="">2021-02-05T07:55:55Z</dcterms:available> <dspace:hasBitstream rdf:resource=""/> <dcterms:rights rdf:resource=""/> <dc:creator>Anderson, J. L. Ross</dc:creator> <dc:contributor>Perriman, Adam W.</dc:contributor> <dc:contributor>Tang, Tsing-Young Dora</dc:contributor> <dc:contributor>Ting, Valeska P.</dc:contributor> <dc:creator>Rambo, Robert P.</dc:creator> <dc:contributor>Anderson, J. L. Ross</dc:contributor> <dcterms:issued>2020-12-09</dcterms:issued> <dc:contributor>Le Vay, Kristian</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Smith, Andrew J.</dc:contributor> <dc:creator>Carter, Ben M.</dc:creator> <dc:contributor>Carter, Ben M.</dc:contributor> <bibo:uri rdf:resource=""/> <dcterms:title>Controlling Protein Nanocage Assembly with Hydrostatic Pressure</dcterms:title> <dc:creator>Watkins, Daniel W.</dc:creator> <dc:creator>Le Vay, Kristian</dc:creator> <dspace:isPartOfCollection rdf:resource=""/> <dcterms:isPartOf rdf:resource=""/> <dc:creator>Cölfen, Helmut</dc:creator> <dc:contributor>Watkins, Daniel W.</dc:contributor> <dc:creator>Ting, Valeska P.</dc:creator> <dc:date rdf:datatype="">2021-02-05T07:55:55Z</dc:date> <dc:creator>Tang, Tsing-Young Dora</dc:creator> <dc:creator>Smith, Andrew J.</dc:creator> <dcterms:abstract xml:lang="eng">Controlling the assembly and disassembly of nanoscale protein cages for the capture and internalization of protein or non-proteinaceous components is fundamentally important to a diverse range of bionanotechnological applications. Here, we study the reversible, pressure-induced dissociation of a natural protein nanocage, E. coli bacterioferritin (Bfr), using synchrotron radiation small-angle X-ray scattering (SAXS) and circular dichroism (CD). We demonstrate that hydrostatic pressures of 450 MPa are sufficient to completely dissociate the Bfr 24-mer into protein dimers, and the reversibility and kinetics of the reassembly process can be controlled by selecting appropriate buffer conditions. We also demonstrate that the heme B prosthetic group present at the subunit dimer interface influences the stability and pressure lability of the cage, despite its location being discrete from the interdimer interface that is key to cage assembly. This indicates a major cage-stabilizing role for heme within this family of ferritins.</dcterms:abstract> <dc:creator>Perriman, Adam W.</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:hasPart rdf:resource=""/> <dc:contributor>Cölfen, Helmut</dc:contributor> <dc:contributor>Rambo, Robert P.</dc:contributor> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

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