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Transient transformation of the obligate biotrophic rust fungus Uromyces fabae using biolistics

Transient transformation of the obligate biotrophic rust fungus Uromyces fabae using biolistics

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DJULIC, Alma, Annette SCHMID, Heike LENZ, Pia SHARMA, Christin KOCH, Stefan G. R. WIRSEL, Ralf T. VOEGELE, 2011. Transient transformation of the obligate biotrophic rust fungus Uromyces fabae using biolistics. In: Fungal Biology. Elsevier. 115(7), pp. 633-642. ISSN 1878-6146. eISSN 1878-6162. Available under: doi: 10.1016/j.funbio.2011.03.007

@article{Djulic2011-07Trans-51228, title={Transient transformation of the obligate biotrophic rust fungus Uromyces fabae using biolistics}, year={2011}, doi={10.1016/j.funbio.2011.03.007}, number={7}, volume={115}, issn={1878-6146}, journal={Fungal Biology}, pages={633--642}, author={Djulic, Alma and Schmid, Annette and Lenz, Heike and Sharma, Pia and Koch, Christin and Wirsel, Stefan G. R. and Voegele, Ralf T.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/51228"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-10-06T12:34:46Z</dc:date> <dc:creator>Djulic, Alma</dc:creator> <dc:contributor>Wirsel, Stefan G. R.</dc:contributor> <dc:contributor>Lenz, Heike</dc:contributor> <dc:contributor>Schmid, Annette</dc:contributor> <dc:creator>Koch, Christin</dc:creator> <dc:creator>Voegele, Ralf T.</dc:creator> <dcterms:title>Transient transformation of the obligate biotrophic rust fungus Uromyces fabae using biolistics</dcterms:title> <dc:contributor>Djulic, Alma</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-10-06T12:34:46Z</dcterms:available> <dc:contributor>Voegele, Ralf T.</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/51228"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:issued>2011-07</dcterms:issued> <dc:creator>Sharma, Pia</dc:creator> <dcterms:abstract xml:lang="eng">Obligate biotrophic pathogens like the rust fungi are important plant pathogens causing enormous losses on food, forage and biomass crops. The analysis of the molecular details underlying obligate biotrophic host–parasite interactions is mainly hampered by the fact that no system for transformation is available for most obligate biotrophic organisms. Here we report the transient transformation of Uromyces fabae, an obligate biotrophic rust fungus using a biolistic approach. Biolistic bombardment of U. fabae urediospores was used to deliver different color markers (β-glucuronidase (GUS), intron green fluorescent protein (iGFP) and red fluorescent protein (DsRed) and/or a selection marker. Endogenous regulatory elements from U. fabae plasma membrane ATPase (Uf-PMA1) were used to drive expression of the transgenes. In addition to the delivery of color markers, an in planta selection procedure using the fungicide Carboxin was established allowing the propagation of transformants. In addition to mere cytoplasmic expression of the color markers, a nuclear localization signal was fused to DsRed (pRV115-NLS) targeting the fluorescent marker protein to the nuclei. A procedure for the genetic modification of U. fabae was established. The method can be easily adapted for use with other obligate biotrophic fungi. This provides the basis for a more in depth analysis of the molecular principles governing the obligate biotrophic lifestyle.</dcterms:abstract> <dc:contributor>Sharma, Pia</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:language>eng</dc:language> <dc:creator>Wirsel, Stefan G. R.</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Koch, Christin</dc:contributor> <dc:creator>Lenz, Heike</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Schmid, Annette</dc:creator> </rdf:Description> </rdf:RDF>

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