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Aqueous extracts of the marine brown alga Lobophora variegata inhibit HIV-1 infection at the level of virus entry into cells

Aqueous extracts of the marine brown alga Lobophora variegata inhibit HIV-1 infection at the level of virus entry into cells

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KREMB, Stephan, Markus HELFER, Birgit KRAUS, Horst WOLFF, Christian WILD, Martha SCHNEIDER, Christian R. VOOLSTRA, Ruth BRACK-WERNER, 2014. Aqueous extracts of the marine brown alga Lobophora variegata inhibit HIV-1 infection at the level of virus entry into cells. In: PloS ONE. Public Library of Science (PLoS). 9(8), e103895. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0103895

@article{Kremb2014Aqueo-50916, title={Aqueous extracts of the marine brown alga Lobophora variegata inhibit HIV-1 infection at the level of virus entry into cells}, year={2014}, doi={10.1371/journal.pone.0103895}, number={8}, volume={9}, journal={PloS ONE}, author={Kremb, Stephan and Helfer, Markus and Kraus, Birgit and Wolff, Horst and Wild, Christian and Schneider, Martha and Voolstra, Christian R. and Brack-Werner, Ruth}, note={Article Number: e103895} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/50916"> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Wolff, Horst</dc:creator> <dcterms:issued>2014</dcterms:issued> <dc:contributor>Helfer, Markus</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-09-21T09:43:34Z</dc:date> <dcterms:abstract xml:lang="eng">In recent years, marine algae have emerged as a rich and promising source of molecules with potent activities against various human pathogens. The widely distributed brown alga Lobophora variegata that is often associated with tropical coral reefs exerts strong antibacterial and antiprotozoal effects, but so far has not been associated with specific anti-viral activities. This study investigated potential HIV-1 inhibitory activity of L. variegata collected from different geographical regions, using a cell-based full replication HIV-1 reporter assay. Aqueous L. variegata extracts showed strong inhibitory effects on several HIV-1 strains, including drug-resistant and primary HIV-1 isolates, and protected even primary cells (PBMC) from HIV-1-infection. Anti-viral potency was related to ecological factors and showed clear differences depending on light exposition or epiphyte growth. Assays addressing early events of the HIV-1 replication cycle indicated that L. variegata extracts inhibited entry of HIV-1 into cells at a pre-fusion step possibly by impeding mobility of virus particles. Further characterization of the aqueous extract demonstrated that even high doses had only moderate effects on viability of cultured and primary cells (PBMCs). Imaging-based techniques revealed extract effects on the plasma membrane and actin filaments as well as induction of apoptosis at concentrations exceeding EC50 of anti-HIV-1 activity by more than 400 fold. In summary, we show for the first time that L. variegata extracts inhibit HIV-1 entry, thereby suggesting this alga as promising source for the development of novel HIV-1 inhibitors.</dcterms:abstract> <dc:creator>Brack-Werner, Ruth</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Schneider, Martha</dc:creator> <dc:rights>Attribution 4.0 International</dc:rights> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-09-21T09:43:34Z</dcterms:available> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/50916/3/Kremb_2-sdrw1fyees2x0.pdf"/> <dc:creator>Kremb, Stephan</dc:creator> <dc:creator>Wild, Christian</dc:creator> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/50916/3/Kremb_2-sdrw1fyees2x0.pdf"/> <dc:contributor>Kremb, Stephan</dc:contributor> <dc:creator>Helfer, Markus</dc:creator> <dc:contributor>Schneider, Martha</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Wild, Christian</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Kraus, Birgit</dc:contributor> <dc:contributor>Voolstra, Christian R.</dc:contributor> <dc:contributor>Brack-Werner, Ruth</dc:contributor> <dcterms:title>Aqueous extracts of the marine brown alga Lobophora variegata inhibit HIV-1 infection at the level of virus entry into cells</dcterms:title> <dc:creator>Voolstra, Christian R.</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/50916"/> <dc:creator>Kraus, Birgit</dc:creator> <dc:contributor>Wolff, Horst</dc:contributor> </rdf:Description> </rdf:RDF>

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