KOPS - The Institutional Repository of the University of Konstanz

Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts

Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts

Cite This

Files in this item

Checksum: MD5:654d79c660118a2c3c0aeeeb53bfdc09

LEVIN, Rachel A., Christian R. VOOLSTRA, Shobhit AGRAWAL, Peter D. STEINBERG, David J. SUGGETT, Madeleine J. H. VAN OPPEN, 2017. Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts. In: Frontiers in Microbiology. Frontiers Media. 8, 1220. eISSN 1664-302X. Available under: doi: 10.3389/fmicb.2017.01220

@article{Levin2017Engin-50767, title={Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts}, year={2017}, doi={10.3389/fmicb.2017.01220}, volume={8}, journal={Frontiers in Microbiology}, author={Levin, Rachel A. and Voolstra, Christian R. and Agrawal, Shobhit and Steinberg, Peter D. and Suggett, David J. and van Oppen, Madeleine J. H.}, note={Article Number: 1220} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/50767"> <dc:creator>Voolstra, Christian R.</dc:creator> <dc:contributor>Steinberg, Peter D.</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Steinberg, Peter D.</dc:creator> <dcterms:title>Engineering Strategies to Decode and Enhance the Genomes of Coral Symbionts</dcterms:title> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/50767"/> <dc:creator>van Oppen, Madeleine J. H.</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Suggett, David J.</dc:creator> <dc:rights>Attribution 4.0 International</dc:rights> <dc:contributor>Agrawal, Shobhit</dc:contributor> <dc:contributor>Suggett, David J.</dc:contributor> <dc:contributor>Levin, Rachel A.</dc:contributor> <dc:creator>Levin, Rachel A.</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/50767/3/Levin_2-1vqn1al86hoj07.pdf"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-09-10T08:53:10Z</dcterms:available> <dc:contributor>van Oppen, Madeleine J. H.</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-09-10T08:53:10Z</dc:date> <dc:creator>Agrawal, Shobhit</dc:creator> <dcterms:abstract xml:lang="eng">Elevated sea surface temperatures from a severe and prolonged El Niño event (2014-2016) fueled by climate change have resulted in mass coral bleaching (loss of dinoflagellate photosymbionts, Symbiodinium spp., from coral tissues) and subsequent coral mortality, devastating reefs worldwide. Genetic variation within and between Symbiodinium species strongly influences the bleaching tolerance of corals, thus recent papers have called for genetic engineering of Symbiodinium to elucidate the genetic basis of bleaching-relevant Symbiodinium traits. However, while Symbiodinium has been intensively studied for over 50 years, genetic transformation of Symbiodinium has seen little success likely due to the large evolutionary divergence between Symbiodinium and other model eukaryotes rendering standard transformation systems incompatible. Here, we integrate the growing wealth of Symbiodinium next-generation sequencing data to design tailored genetic engineering strategies. Specifically, we develop a testable expression construct model that incorporates endogenous Symbiodinium promoters, terminators, and genes of interest, as well as an internal ribosomal entry site from a Symbiodinium virus. Furthermore, we assess the potential for CRISPR/Cas9 genome editing through new analyses of the three currently available Symbiodinium genomes. Finally, we discuss how genetic engineering could be applied to enhance the stress tolerance of Symbiodinium, and in turn, coral reefs.</dcterms:abstract> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/50767/3/Levin_2-1vqn1al86hoj07.pdf"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:issued>2017</dcterms:issued> <dc:contributor>Voolstra, Christian R.</dc:contributor> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

Downloads since Sep 10, 2020 (Information about access statistics)

Levin_2-1vqn1al86hoj07.pdf 189

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


Browse

My Account