Structural correlations and melting of B-DNA fibers
| Type of Publication: | Journal article |
| Publication status: | Published |
| Author: | Wildes, Andrew; Theodorakopoulos, Nikos; Valle-Orero, Jessica; Cuesta-López, Santiago; Garden, Jean-Luc; Peyrard, Michel |
| Year of publication: | 2011 |
| Published in: | Physical Review E ; 83 (2011), 6. - 061923. - American Physical Society (APS). - ISSN 1539-3755. - eISSN 1550-2376 |
| Pubmed ID: | 21797419 |
| DOI (citable link): | https://dx.doi.org/10.1103/PhysRevE.83.061923 |
| Summary: |
Despite numerous attempts, understanding the thermal denaturation of DNA is still a challenge due to the lack of structural data on the transition since standard experimental approaches to DNA melting are made in solution and do not provide spatial information. We report a measurement using neutron scattering from oriented DNA fibers to determine the size of the regions that stay in the double-helix conformation as the melting temperature is approached from below. A Bragg peak from the B form of DNA is observed as a function of temperature and its width and integrated intensity are measured. These results, complemented by a differential calorimetry study of the melting of B-DNA fibers as well as electrophoresis and optical observation data, are analyzed in terms of a one-dimensional mesoscopic model of DNA.
|
| Subject (DDC): | 530 Physics |
| Bibliography of Konstanz: | Yes |
| Refereed: | Yes |
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WILDES, Andrew, Nikos THEODORAKOPOULOS, Jessica VALLE-ORERO, Santiago CUESTA-LÓPEZ, Jean-Luc GARDEN, Michel PEYRARD, 2011. Structural correlations and melting of B-DNA fibers. In: Physical Review E. American Physical Society (APS). 83(6), 061923. ISSN 1539-3755. eISSN 1550-2376. Available under: doi: 10.1103/PhysRevE.83.061923
@article{Wildes2011-06Struc-50375, title={Structural correlations and melting of B-DNA fibers}, year={2011}, doi={10.1103/PhysRevE.83.061923}, number={6}, volume={83}, issn={1539-3755}, journal={Physical Review E}, author={Wildes, Andrew and Theodorakopoulos, Nikos and Valle-Orero, Jessica and Cuesta-López, Santiago and Garden, Jean-Luc and Peyrard, Michel}, note={Article Number: 061923} }
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