Type of Publication: | Journal article |
Publication status: | Published |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-2-1v4iz900qs99f8 |
Author: | Schindl, Kathrin; Sharma, Deepika; Spiteller, Dieter |
Year of publication: | 2020 |
Published in: | ChemBioChem ; 21 (2020), 21. - pp. 3151-3157. - Wiley. - ISSN 1439-4227. - eISSN 1439-7633 |
Pubmed ID: | 32585063 |
DOI (citable link): | https://dx.doi.org/10.1002/cbic.202000404 |
Summary: |
When Streptomyces violaceoruber grows together with Streptomyces sp. MG7-G1, it reacts with strongly induced droplet production on its aerial mycelium. Initially the metabolite profile of droplets from S. violaceoruber in co-culture with Streptomyces sp. MG7-G1 was compared to samples from S. violaceoruber in single-culture using high-performance liquid chromatography-mass spectrometry (HPLC-MS). Then, also the exudate from agar plates of co-cultures and single cultures (after freezing and thawing) was analysed. Several compounds were only observed when S. violaceoruber was grown in co-culture. Based on their high-resolution ESI mass spectra and their comparable retention times to the calcium-dependent antibiotics (CDAs) produced by S. violaceo-ruber the new compounds were suspected to be deacylated calcium-dependent antibiotics (daCDAs), lacking the 2,3-epoxyhexanoyl residue of CDAs. This was verified by detailed analysis of the MS/MS spectra of the deacylated CDAs in comparison to the CDAs. The major CDA compounds present in calcium ion supplemented agar medium of co-cultures were daCDAs, suggesting that Streptomyces sp. MG7-G1 expresses a deacylase that degrades CDAs.
|
Subject (DDC): | 570 Biosciences, Biology |
Keywords: | antibiotic, lipopeptide, mass spectrometry, microbe-microbe interactions, natural product |
Link to License: | In Copyright |
Bibliography of Konstanz: | Yes |
Refereed: | Yes |
SCHINDL, Kathrin, Deepika SHARMA, Dieter SPITELLER, 2020. Deacylation of calcium-dependent antibiotics from Streptomyces violaceoruber in co-culture with Streptomyces sp. MG7-G1. In: ChemBioChem. Wiley. 21(21), pp. 3151-3157. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.202000404
@article{Schindl2020-11-02Deacy-50082, title={Deacylation of calcium-dependent antibiotics from Streptomyces violaceoruber in co-culture with Streptomyces sp. MG7-G1}, year={2020}, doi={10.1002/cbic.202000404}, number={21}, volume={21}, issn={1439-4227}, journal={ChemBioChem}, pages={3151--3157}, author={Schindl, Kathrin and Sharma, Deepika and Spiteller, Dieter} }
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/50082"> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:contributor>Schindl, Kathrin</dc:contributor> <dc:contributor>Spiteller, Dieter</dc:contributor> <dc:contributor>Sharma, Deepika</dc:contributor> <dcterms:title>Deacylation of calcium-dependent antibiotics from Streptomyces violaceoruber in co-culture with Streptomyces sp. MG7-G1</dcterms:title> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Sharma, Deepika</dc:creator> <dcterms:issued>2020-11-02</dcterms:issued> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/50082"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Spiteller, Dieter</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/50082/1/Schindl_2-1v4iz900qs99f8.pdf"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-07-01T11:03:13Z</dc:date> <dc:creator>Schindl, Kathrin</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-07-01T11:03:13Z</dcterms:available> <dc:rights>terms-of-use</dc:rights> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:abstract xml:lang="eng">When Streptomyces violaceoruber grows together with Streptomyces sp. MG7-G1, it reacts with strongly induced droplet production on its aerial mycelium. Initially the metabolite profile of droplets from S. violaceoruber in co-culture with Streptomyces sp. MG7-G1 was compared to samples from S. violaceoruber in single-culture using high-performance liquid chromatography-mass spectrometry (HPLC-MS). Then, also the exudate from agar plates of co-cultures and single cultures (after freezing and thawing) was analysed. Several compounds were only observed when S. violaceoruber was grown in co-culture. Based on their high-resolution ESI mass spectra and their comparable retention times to the calcium-dependent antibiotics (CDAs) produced by S. violaceo-ruber the new compounds were suspected to be deacylated calcium-dependent antibiotics (daCDAs), lacking the 2,3-epoxyhexanoyl residue of CDAs. This was verified by detailed analysis of the MS/MS spectra of the deacylated CDAs in comparison to the CDAs. The major CDA compounds present in calcium ion supplemented agar medium of co-cultures were daCDAs, suggesting that Streptomyces sp. MG7-G1 expresses a deacylase that degrades CDAs.</dcterms:abstract> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/50082/1/Schindl_2-1v4iz900qs99f8.pdf"/> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>
Schindl_2-1v4iz900qs99f8.pdf | 50 |