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Protegrin-1 cytotoxicity towards mammalian cells positively correlates with the magnitude of conformational changes of the unfolded form upon cell interaction

Protegrin-1 cytotoxicity towards mammalian cells positively correlates with the magnitude of conformational changes of the unfolded form upon cell interaction

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SOUNDRARAJAN, Nagasundarapandian, Suhyun PARK, Quy LE VAN CHANH, Hye-Sun CHO, Govindan RAGHUNATHAN, Byeongyong AHN, Hyuk SONG, Jin-Hoi KIM, Chankyu PARK, 2019. Protegrin-1 cytotoxicity towards mammalian cells positively correlates with the magnitude of conformational changes of the unfolded form upon cell interaction. In: Scientific reports. 9(1), 11569. eISSN 2045-2322. Available under: doi: 10.1038/s41598-019-47955-2

@article{Soundrarajan2019-08-09Prote-48022, title={Protegrin-1 cytotoxicity towards mammalian cells positively correlates with the magnitude of conformational changes of the unfolded form upon cell interaction}, year={2019}, doi={10.1038/s41598-019-47955-2}, number={1}, volume={9}, journal={Scientific reports}, author={Soundrarajan, Nagasundarapandian and Park, Suhyun and Le Van Chanh, Quy and Cho, Hye-Sun and Raghunathan, Govindan and Ahn, Byeongyong and Song, Hyuk and Kim, Jin-Hoi and Park, Chankyu}, note={Article Number: 11569} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/48022"> <dc:contributor>Park, Chankyu</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-12-12T14:25:27Z</dcterms:available> <dc:contributor>Raghunathan, Govindan</dc:contributor> <dc:creator>Park, Chankyu</dc:creator> <dc:creator>Song, Hyuk</dc:creator> <dcterms:title>Protegrin-1 cytotoxicity towards mammalian cells positively correlates with the magnitude of conformational changes of the unfolded form upon cell interaction</dcterms:title> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:creator>Ahn, Byeongyong</dc:creator> <dc:creator>Le Van Chanh, Quy</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/48022"/> <dc:creator>Kim, Jin-Hoi</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/48022/1/Soundrarajan_2-1n72j4tdafvr21.pdf"/> <dc:creator>Raghunathan, Govindan</dc:creator> <dc:creator>Park, Suhyun</dc:creator> <dc:contributor>Le Van Chanh, Quy</dc:contributor> <dc:contributor>Kim, Jin-Hoi</dc:contributor> <dcterms:abstract xml:lang="eng">Porcine protegrin-1 (PG-1) is a broad-spectrum antimicrobial peptide (AMP) with potent antimicrobial activities. We produced recombinant PG-1 and evaluated its cytotoxicity toward various types of mammalian cell lines, including embryonic fibroblasts, retinal cells, embryonic kidney cells, neuroblastoma cells, alveolar macrophage cells, and neutrophils. The sensitivity of the different mammalian cells to cytotoxic damage induced by PG-1 differed significantly among the cell types, with retinal neuron cells and neutrophils being the most significantly affected. A circular dichroism analysis showed there was a precise correlation between conformational changes in PG-1 and the magnitude of cytotoxicity among the various cell type. Subsequently, a green fluorescent protein (GFP) penetration assay using positively charged GFPs indicated there was a close correlation between the degree of penetration of charged GFP into cells and the magnitude of PG-1 cytotoxicity. Furthermore, we also showed that inhibition of the synthesis of anionic sulphated proteoglycans on the cell surface decreases the cytotoxic damage induced by PG-1 treatment. Taken together, the observed cytotoxicity of PG-1 towards different membrane surfaces is highly driven by the membrane's anionic properties. Our results reveal a possible mechanism underlying cell-type dependent differences in cytotoxicity of AMPs, such as PG-1, toward mammalian cells.</dcterms:abstract> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/48022/1/Soundrarajan_2-1n72j4tdafvr21.pdf"/> <dc:rights>terms-of-use</dc:rights> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:language>eng</dc:language> <dc:creator>Soundrarajan, Nagasundarapandian</dc:creator> <dc:contributor>Cho, Hye-Sun</dc:contributor> <dc:contributor>Song, Hyuk</dc:contributor> <dc:contributor>Park, Suhyun</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-12-12T14:25:27Z</dc:date> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:creator>Cho, Hye-Sun</dc:creator> <dc:contributor>Ahn, Byeongyong</dc:contributor> <dcterms:issued>2019-08-09</dcterms:issued> <dc:contributor>Soundrarajan, Nagasundarapandian</dc:contributor> </rdf:Description> </rdf:RDF>

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