KOPS - The Institutional Repository of the University of Konstanz

A single Thaumarchaeon drives nitrification in deep oligotrophic Lake Constance

A single Thaumarchaeon drives nitrification in deep oligotrophic Lake Constance

Cite This

Files in this item

Checksum: MD5:3e6d6dc5aa6a54044998d0d9cbb4dc85

HERBER, Janina, Franziska KLOTZ, Benjamin FROMMEYER, Severin WEIS, Dietmar STRAILE, Allison KOLAR, Johannes SIKORSKI, Markus EGERT, Michael DANNENMANN, Michael PESTER, 2020. A single Thaumarchaeon drives nitrification in deep oligotrophic Lake Constance. In: Environmental Microbiology. Wiley. 22(1), pp. 212-228. ISSN 1462-2912. eISSN 1462-2920. Available under: doi: 10.1111/1462-2920.14840

@article{Herber2020-01singl-47665, title={A single Thaumarchaeon drives nitrification in deep oligotrophic Lake Constance}, year={2020}, doi={10.1111/1462-2920.14840}, number={1}, volume={22}, issn={1462-2912}, journal={Environmental Microbiology}, pages={212--228}, author={Herber, Janina and Klotz, Franziska and Frommeyer, Benjamin and Weis, Severin and Straile, Dietmar and Kolar, Allison and Sikorski, Johannes and Egert, Markus and Dannenmann, Michael and Pester, Michael} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/47665"> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Pester, Michael</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-11-27T13:56:26Z</dc:date> <dc:creator>Frommeyer, Benjamin</dc:creator> <dc:language>eng</dc:language> <dc:creator>Herber, Janina</dc:creator> <dc:contributor>Weis, Severin</dc:contributor> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:creator>Straile, Dietmar</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/47665/1/Herber_2-u7bsgohkhwlr3.pdf"/> <dc:contributor>Klotz, Franziska</dc:contributor> <dc:creator>Klotz, Franziska</dc:creator> <dc:contributor>Egert, Markus</dc:contributor> <dc:contributor>Kolar, Allison</dc:contributor> <dc:contributor>Straile, Dietmar</dc:contributor> <dc:creator>Egert, Markus</dc:creator> <dc:contributor>Frommeyer, Benjamin</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Weis, Severin</dc:creator> <dc:contributor>Herber, Janina</dc:contributor> <dc:creator>Pester, Michael</dc:creator> <dcterms:abstract xml:lang="eng">Ammonia released during organic matter mineralization is converted during nitrification to nitrate. We followed spatiotemporal dynamics of the nitrifying microbial community in deep oligotrophic Lake Constance. Depth‐dependent decrease of total ammonium (0.01–0.84 μM) indicated the hypolimnion as the major place of nitrification with 15N‐isotope dilution measurements indicating a threefold daily turnover of hypolimnetic total ammonium. This was mirrored by a strong increase of ammonia‐oxidizing Thaumarchaeota towards the hypolimnion (13%–21% of bacterioplankton) throughout spring to autumn as revealed by amplicon sequencing and quantitative polymerase chain reaction. Ammonia‐oxidizing bacteria were typically two orders of magnitude less abundant and completely ammonia‐oxidizing (comammox) bacteria were not detected. Both, 16S rRNA gene and amoA (encoding ammonia monooxygenase subunit B) analyses identified only one major species‐level operational taxonomic unit (OTU) of Thaumarchaeota (99% of all ammonia oxidizers in the hypolimnion), which was affiliated to Nitrosopumilus spp. The relative abundance distribution of the single Thaumarchaeon strongly correlated to an equally abundant Chloroflexi clade CL500‐11 OTU and a Nitrospira OTU that was one order of magnitude less abundant. The latter dominated among recognized nitrite oxidizers. This extremely low diversity of nitrifiers shows how vulnerable the ecosystem process of nitrification may be in Lake Constance as Central Europe's third largest lake.</dcterms:abstract> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/47665"/> <dcterms:title>A single Thaumarchaeon drives nitrification in deep oligotrophic Lake Constance</dcterms:title> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/47665/1/Herber_2-u7bsgohkhwlr3.pdf"/> <dc:contributor>Sikorski, Johannes</dc:contributor> <dc:creator>Dannenmann, Michael</dc:creator> <dc:contributor>Dannenmann, Michael</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-11-27T13:56:26Z</dcterms:available> <dcterms:issued>2020-01</dcterms:issued> <dc:creator>Sikorski, Johannes</dc:creator> <dc:creator>Kolar, Allison</dc:creator> </rdf:Description> </rdf:RDF>

Downloads since Nov 27, 2019 (Information about access statistics)

Herber_2-u7bsgohkhwlr3.pdf 29

This item appears in the following Collection(s)

Search KOPS


Browse

My Account