KOPS - The Institutional Repository of the University of Konstanz

Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne Bearing Noncanonical Amino Acids

Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne Bearing Noncanonical Amino Acids

Cite This

Files in this item

Checksum: MD5:4aacda9684659250f5b9aa38d42c501d

BRAUN, Theresa Sophie, Pia WIDDER, Uwe OSSWALD, Lina GROSS, Lara WILLIAMS, Moritz SCHMIDT, Irina HELMLE, Daniel SUMMERER, Malte DRESCHER, 2020. Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne Bearing Noncanonical Amino Acids. In: ChemBioChem. Wiley. 21(7), pp. 958-962. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201900537

@article{Braun2020-04Isoin-47475, title={Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne Bearing Noncanonical Amino Acids}, year={2020}, doi={10.1002/cbic.201900537}, number={7}, volume={21}, issn={1439-4227}, journal={ChemBioChem}, pages={958--962}, author={Braun, Theresa Sophie and Widder, Pia and Osswald, Uwe and Groß, Lina and Williams, Lara and Schmidt, Moritz and Helmle, Irina and Summerer, Daniel and Drescher, Malte} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/47475"> <dc:contributor>Schmidt, Moritz</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/47475/1/Braun_2-1cspjvat5sbol6.pdf"/> <dc:contributor>Drescher, Malte</dc:contributor> <dc:creator>Groß, Lina</dc:creator> <dc:contributor>Braun, Theresa Sophie</dc:contributor> <dc:contributor>Williams, Lara</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/47475/1/Braun_2-1cspjvat5sbol6.pdf"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Summerer, Daniel</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-11-13T10:00:26Z</dc:date> <dc:creator>Drescher, Malte</dc:creator> <dc:creator>Williams, Lara</dc:creator> <dcterms:abstract xml:lang="eng">Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling (SDSL) is a powerful tool in protein structural research. Nitroxides are highly suitable spin labeling reagents, but suffer from limited stability in particular in cellular environment. Here, we present the synthesis of a maleimid- and an azide-modified tetraethyl-shielded isoindoline-based nitroxide (M- and Az-TEIO) for labeling of cysteines or the noncanonical amino acid para -ethynyl-l-phenylalanine ( p ENF). We demonstrate high stability of TEIO  site-specifically attached to the protein thioredoxin (TRX) towards reduction in prokaryotic and eukaryotic environments, and conduct double electron-electron resonance (DEER) measurements. We further generate a rotamer library for the new residue p ENF-Az-TEIO that affords a distance distribution that is in agreement with the measured distribution.</dcterms:abstract> <dc:contributor>Widder, Pia</dc:contributor> <dc:creator>Widder, Pia</dc:creator> <dc:creator>Helmle, Irina</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/47475"/> <dc:creator>Braun, Theresa Sophie</dc:creator> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Summerer, Daniel</dc:contributor> <dc:language>eng</dc:language> <dcterms:title>Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne Bearing Noncanonical Amino Acids</dcterms:title> <dc:contributor>Osswald, Uwe</dc:contributor> <dcterms:issued>2020-04</dcterms:issued> <dc:contributor>Helmle, Irina</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-11-13T10:00:26Z</dcterms:available> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:creator>Osswald, Uwe</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:contributor>Groß, Lina</dc:contributor> <dc:creator>Schmidt, Moritz</dc:creator> </rdf:Description> </rdf:RDF>

Downloads since Nov 13, 2019 (Information about access statistics)

Braun_2-1cspjvat5sbol6.pdf 18

This item appears in the following Collection(s)

Search KOPS


Browse

My Account