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Characterization of Drosophila Nidogen/entactin reveals roles in basement membrane stability, barrier function and nervous system patterning

Characterization of Drosophila Nidogen/entactin reveals roles in basement membrane stability, barrier function and nervous system patterning

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WOLFSTETTER, Georg, Ina DAHLITZ, Kathrin PFEIFER, Uwe TÖPFER, Joscha Arne ALT, Daniel Christoph PFEIFER, Reinhard LAKES-HARLAN, Stefan BAUMGARTNER, Ruth H. PALMER, Anne HOLZ, 2019. Characterization of Drosophila Nidogen/entactin reveals roles in basement membrane stability, barrier function and nervous system patterning. In: Development. 146(2), dev168948. ISSN 0022-0752. eISSN 1477-9129. Available under: doi: 10.1242/dev.168948

@article{Wolfstetter2019-01-16Chara-46707, title={Characterization of Drosophila Nidogen/entactin reveals roles in basement membrane stability, barrier function and nervous system patterning}, year={2019}, doi={10.1242/dev.168948}, number={2}, volume={146}, issn={0022-0752}, journal={Development}, author={Wolfstetter, Georg and Dahlitz, Ina and Pfeifer, Kathrin and Töpfer, Uwe and Alt, Joscha Arne and Pfeifer, Daniel Christoph and Lakes-Harlan, Reinhard and Baumgartner, Stefan and Palmer, Ruth H. and Holz, Anne}, note={Article Number: dev168948} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/46707"> <dc:creator>Baumgartner, Stefan</dc:creator> <dc:contributor>Pfeifer, Daniel Christoph</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/46707"/> <dc:contributor>Holz, Anne</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Wolfstetter, Georg</dc:contributor> <dc:creator>Palmer, Ruth H.</dc:creator> <dc:creator>Pfeifer, Kathrin</dc:creator> <dc:contributor>Lakes-Harlan, Reinhard</dc:contributor> <dc:creator>Wolfstetter, Georg</dc:creator> <dc:creator>Holz, Anne</dc:creator> <dcterms:abstract xml:lang="eng">Basement membranes (BMs) are specialized layers of extracellular matrix (ECM) mainly composed of Laminin, type IV Collagen, Perlecan and Nidogen/entactin (NDG). Recent in vivo studies challenged the initially proposed role of NDG as a major ECM linker molecule by revealing dispensability for viability and BM formation. Here, we report the characterization of the single Ndg gene in Drosophila. Embryonic Ndg expression was primarily observed in mesodermal tissues and the chordotonal organs, whereas NDG protein localized to all BMs. Although loss of Laminin strongly affected BM localization of NDG, Ndg-null mutants exhibited no overt changes in the distribution of BM components. Although Drosophila Ndg mutants were viable, loss of NDG led to ultrastructural BM defects that compromised barrier function and stability in vivo Moreover, loss of NDG impaired larval crawling behavior and reduced responses to vibrational stimuli. Further morphological analysis revealed accompanying defects in the larval peripheral nervous system, especially in the chordotonal organs and the neuromuscular junction (NMJ). Taken together, our analysis suggests that NDG is not essential for BM assembly but mediates BM stability and ECM-dependent neural plasticity during Drosophila development.</dcterms:abstract> <dc:creator>Dahlitz, Ina</dc:creator> <dc:contributor>Baumgartner, Stefan</dc:contributor> <dcterms:issued>2019-01-16</dcterms:issued> <dc:creator>Lakes-Harlan, Reinhard</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-08-21T11:17:48Z</dc:date> <dc:contributor>Pfeifer, Kathrin</dc:contributor> <dc:creator>Pfeifer, Daniel Christoph</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-08-21T11:17:48Z</dcterms:available> <dc:creator>Töpfer, Uwe</dc:creator> <dc:contributor>Dahlitz, Ina</dc:contributor> <dcterms:title>Characterization of Drosophila Nidogen/entactin reveals roles in basement membrane stability, barrier function and nervous system patterning</dcterms:title> <dc:language>eng</dc:language> <dc:contributor>Töpfer, Uwe</dc:contributor> <dc:creator>Alt, Joscha Arne</dc:creator> <dc:contributor>Palmer, Ruth H.</dc:contributor> <dc:contributor>Alt, Joscha Arne</dc:contributor> </rdf:Description> </rdf:RDF>

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