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Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC

Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC

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GAMERDINGER, Martin, Kan KOBAYASHI, Annalena WALLISCH, Stefan G. KREFT, Carolin SAILER, Renate SCHLÖMER, Nadine SACHS, Florian STENGEL, Nenad BAN, Elke DEUERLING, 2019. Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC. In: Molecular Cell. 75(5), pp. 996-1006.e8. ISSN 1097-2765. eISSN 1097-4164. Available under: doi: 10.1016/j.molcel.2019.06.030

@article{Gamerdinger2019-09Early-46661, title={Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC}, year={2019}, doi={10.1016/j.molcel.2019.06.030}, number={5}, volume={75}, issn={1097-2765}, journal={Molecular Cell}, pages={996--1006.e8}, author={Gamerdinger, Martin and Kobayashi, Kan and Wallisch, Annalena and Kreft, Stefan G. and Sailer, Carolin and Schlömer, Renate and Sachs, Nadine and Stengel, Florian and Ban, Nenad and Deuerling, Elke} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/46661"> <dc:creator>Deuerling, Elke</dc:creator> <dc:creator>Kreft, Stefan G.</dc:creator> <dc:creator>Sachs, Nadine</dc:creator> <dc:contributor>Schlömer, Renate</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/46661"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Wallisch, Annalena</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-08-08T15:04:56Z</dc:date> <dcterms:title>Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC</dcterms:title> <dc:creator>Ban, Nenad</dc:creator> <dc:contributor>Sailer, Carolin</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Gamerdinger, Martin</dc:creator> <dc:contributor>Stengel, Florian</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Sailer, Carolin</dc:creator> <dc:creator>Kobayashi, Kan</dc:creator> <dc:creator>Schlömer, Renate</dc:creator> <dc:creator>Stengel, Florian</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Kobayashi, Kan</dc:contributor> <dc:contributor>Kreft, Stefan G.</dc:contributor> <dc:creator>Wallisch, Annalena</dc:creator> <dc:contributor>Ban, Nenad</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-08-08T15:04:56Z</dcterms:available> <dc:contributor>Gamerdinger, Martin</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Sachs, Nadine</dc:contributor> <dc:contributor>Deuerling, Elke</dc:contributor> <dcterms:abstract xml:lang="eng">Cotranslational processing of newly synthesized proteins is fundamental for correct protein maturation. Protein biogenesis factors are thought to bind nascent polypeptides not before they exit the ribosomal tunnel. Here, we identify a nascent chain recognition mechanism deep inside the ribosomal tunnel by an essential eukaryotic cytosolic chaperone. The nascent polypeptide-associated complex (NAC) inserts the N-terminal tail of its β subunit (N-βNAC) into the ribosomal tunnel to sense substrates directly upon synthesis close to the peptidyl-transferase center. N-βNAC escorts the growing polypeptide to the cytosol and relocates to an alternate binding site on the ribosomal surface. Using C. elegans as an in vivo model, we demonstrate that the tunnel-probing activity of NAC is essential for organismal viability and critical to regulate endoplasmic reticulum (ER) protein transport by controlling ribosome-Sec61 translocon interactions. Thus, eukaryotic protein maturation relies on the early sampling of nascent chains inside the ribosomal tunnel.</dcterms:abstract> <dcterms:issued>2019-09</dcterms:issued> </rdf:Description> </rdf:RDF>

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