KOPS - The Institutional Repository of the University of Konstanz

Neomycin-dependent hammerhead ribozymes for the direct control of gene expression in Saccharomyces cerevisiae

Neomycin-dependent hammerhead ribozymes for the direct control of gene expression in Saccharomyces cerevisiae

Cite This

Files in this item

Files Size Format View

There are no files associated with this item.

SACK, Monika, Julia STIFEL, Stefan G. KREFT, Elke DEUERLING, Jörg S. HARTIG, 2019. Neomycin-dependent hammerhead ribozymes for the direct control of gene expression in Saccharomyces cerevisiae. In: Methods. ISSN 1046-2023. eISSN 1095-9130. Available under: doi: 10.1016/j.ymeth.2019.01.004

@article{Sack2019-01-09Neomy-44869, title={Neomycin-dependent hammerhead ribozymes for the direct control of gene expression in Saccharomyces cerevisiae}, year={2019}, doi={10.1016/j.ymeth.2019.01.004}, issn={1046-2023}, journal={Methods}, author={Sack, Monika and Stifel, Julia and Kreft, Stefan G. and Deuerling, Elke and Hartig, Jörg S.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/44869"> <dc:creator>Stifel, Julia</dc:creator> <dc:creator>Hartig, Jörg S.</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-02-06T08:54:05Z</dcterms:available> <dcterms:title>Neomycin-dependent hammerhead ribozymes for the direct control of gene expression in Saccharomyces cerevisiae</dcterms:title> <dc:contributor>Kreft, Stefan G.</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:contributor>Hartig, Jörg S.</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-02-06T08:54:05Z</dc:date> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:abstract xml:lang="eng">Hammerhead ribozyme-based RNA switches have been proven to be powerful tools for conditional gene regulation in various organisms. We present neomycin-dependent hammerhead ribozymes (HHR) that influence gene expression in a ligand- and dose-dependent manner in S. cerevisiae. We utilized a novel design of fusing the aptamer domain to the HHR enabling for the first time the identification of genetic ON- and OFF-switches within the same library. For this purpose a neomycin aptamer was fused to stem 1 of a type 3 hammerhead ribozyme via an addressable three-way junction that shows high flexibility at the connection site. An in vivo screening approach identified sequences that allow to induce or repress gene expression 2- to 3-fold in response to neomycin addition. The ribozyme switches operate at neomycin concentrations that show no toxic effect on cell growth and pose powerful genetic tools to study and modulate cellular function in yeast.</dcterms:abstract> <dc:language>eng</dc:language> <dcterms:issued>2019-01-09</dcterms:issued> <dc:contributor>Sack, Monika</dc:contributor> <dc:contributor>Deuerling, Elke</dc:contributor> <dc:creator>Kreft, Stefan G.</dc:creator> <dc:creator>Deuerling, Elke</dc:creator> <dc:contributor>Stifel, Julia</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/44869"/> <dc:creator>Sack, Monika</dc:creator> </rdf:Description> </rdf:RDF>

This item appears in the following Collection(s)

Search KOPS


Browse

My Account