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Anaerobic Degradation of the Plant Sugar Sulfoquinovose Concomitant With H<sub>2</sub>S Production : Escherichia coli K-12 and Desulfovibrio sp. Strain DF1 as Co-culture Model

Anaerobic Degradation of the Plant Sugar Sulfoquinovose Concomitant With H2S Production : Escherichia coli K-12 and Desulfovibrio sp. Strain DF1 as Co-culture Model

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BURRICHTER, Anna Georga, Karin DENGER, Paolo FRANCHINI, Thomas HUHN, Nicolai MÜLLER, Dieter SPITELLER, David SCHLEHECK, 2018. Anaerobic Degradation of the Plant Sugar Sulfoquinovose Concomitant With H2S Production : Escherichia coli K-12 and Desulfovibrio sp. Strain DF1 as Co-culture Model. In: Frontiers in microbiology. 9, 2792. eISSN 1664-302X. Available under: doi: 10.3389/fmicb.2018.02792

@article{Burrichter2018Anaer-44589, title={Anaerobic Degradation of the Plant Sugar Sulfoquinovose Concomitant With H2S Production : Escherichia coli K-12 and Desulfovibrio sp. Strain DF1 as Co-culture Model}, year={2018}, doi={10.3389/fmicb.2018.02792}, volume={9}, journal={Frontiers in microbiology}, author={Burrichter, Anna Georga and Denger, Karin and Franchini, Paolo and Huhn, Thomas and Müller, Nicolai and Spiteller, Dieter and Schleheck, David}, note={Article Number: 2792} }

Huhn, Thomas Denger, Karin Anaerobic Degradation of the Plant Sugar Sulfoquinovose Concomitant With H<sub>2</sub>S Production : Escherichia coli K-12 and Desulfovibrio sp. Strain DF1 as Co-culture Model Burrichter, Anna Georga Müller, Nicolai 2018 2019-01-16T11:29:59Z 2019-01-16T11:29:59Z eng Sulfoquinovose (SQ, 6-deoxy-6-sulfoglucose) is produced by plants and other phototrophs and its biodegradation is a relevant component of the biogeochemical carbon and sulfur cycles. SQ is known to be degraded by aerobic bacterial consortia in two tiers via C<sub>3</sub>-organosulfonates as transient intermediates to CO<sub>2</sub>, water and sulfate. In this study, we present a first laboratory model for anaerobic degradation of SQ by bacterial consortia in two tiers to acetate and hydrogen sulfide (H<sub>2</sub>S). For the first tier, SQ-degrading Escherichia coli K-12 was used. It catalyzes the fermentation of SQ to 2,3-dihydroxypropane-1-sulfonate (DHPS), succinate, acetate and formate, thus, a novel type of mixed-acid fermentation. It employs the characterized SQ Embden-Meyerhof-Parnas pathway, as confirmed by mutational and proteomic analyses. For the second tier, a DHPS-degrading Desulfovibrio sp. isolate from anaerobic sewage sludge was used, strain DF1. It catalyzes another novel fermentation, of the DHPS to acetate and H<sub>2</sub>S. Its DHPS desulfonation pathway was identified by differential proteomics and demonstrated by heterologously produced enzymes: DHPS is oxidized via 3-sulfolactaldehyde to 3-sulfolactate (SL) by two NAD<sup>+</sup>-dependent dehydrogenases (DhpA, SlaB); the SL is cleaved by an SL sulfite-lyase known from aerobic bacteria (SuyAB) to pyruvate and sulfite. The pyruvate is oxidized to acetate, while the sulfite is used as electron acceptor in respiration and reduced to H<sub>2</sub>S. In conclusion, anaerobic sulfidogenic SQ degradation was demonstrated as a novel link in the biogeochemical sulfur cycle. SQ is also a constituent of the green-vegetable diet of herbivores and omnivores and H<sub>2</sub>S production in the intestinal microbiome has many recognized and potential contributions to human health and disease. Hence, it is important to examine bacterial SQ degradation also in the human intestinal microbiome, in relation to H<sub>2</sub>S production, dietary conditions and human health. Franchini, Paolo Spiteller, Dieter Franchini, Paolo Spiteller, Dieter Müller, Nicolai Denger, Karin Huhn, Thomas Schleheck, David Schleheck, David Burrichter, Anna Georga terms-of-use

Dateiabrufe seit 16.01.2019 (Informationen über die Zugriffsstatistik)

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