Genome editing in diatoms : achievements and goals

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KROTH, Peter G., Atle M. BONES, Fayza DABOUSSI, Maria I. FERRANTE, Marianne JAUBERT, Misha KOLOT, Marianne NYMARK, Carolina RIO BARTULOS, Manuel SERIF, Angela FALCIATORE, 2018. Genome editing in diatoms : achievements and goals. In: Plant Cell Reports. 37(10), pp. 1401-1408. ISSN 0721-7714. eISSN 1432-203X. Available under: doi: 10.1007/s00299-018-2334-1

@article{Kroth2018-10Genom-44586, title={Genome editing in diatoms : achievements and goals}, year={2018}, doi={10.1007/s00299-018-2334-1}, number={10}, volume={37}, issn={0721-7714}, journal={Plant Cell Reports}, pages={1401--1408}, author={Kroth, Peter G. and Bones, Atle M. and Daboussi, Fayza and Ferrante, Maria I. and Jaubert, Marianne and Kolot, Misha and Nymark, Marianne and Rio Bartulos, Carolina and Serif, Manuel and Falciatore, Angela} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:rights>terms-of-use</dc:rights> <dcterms:hasPart rdf:resource=""/> <dcterms:abstract xml:lang="eng">Diatoms are major components of phytoplankton and play a key role in the ecology of aquatic ecosystems. These algae are of great scientific importance for a wide variety of research areas, ranging from marine ecology and oceanography to biotechnology. During the last 20 years, the availability of genomic information on selected diatom species and a substantial progress in genetic manipulation, strongly contributed to establishing diatoms as molecular model organisms for marine biology research. Recently, tailored TALEN endonucleases and the CRISPR/Cas9 system were utilized in diatoms, allowing targeted genetic modifications and the generation of knockout strains. These approaches are extremely valuable for diatom research because breeding, forward genetic screens by random insertion, and chemical mutagenesis are not applicable to the available model species Phaeodactylum tricornutum and Thalassiosira pseudonana, which do not cross sexually in the lab. Here, we provide an overview of the genetic toolbox that is currently available for performing stable genetic modifications in diatoms. We also discuss novel challenges that need to be addressed to fully exploit the potential of these technologies for the characterization of diatom biology and for metabolic engineering.</dcterms:abstract> <dc:contributor>Kroth, Peter G.</dc:contributor> <dc:creator>Nymark, Marianne</dc:creator> <bibo:uri rdf:resource=""/> <dc:contributor>Kolot, Misha</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Serif, Manuel</dc:contributor> <dc:contributor>Bones, Atle M.</dc:contributor> <dcterms:issued>2018-10</dcterms:issued> <dc:creator>Daboussi, Fayza</dc:creator> <dc:contributor>Falciatore, Angela</dc:contributor> <dc:creator>Kroth, Peter G.</dc:creator> <dspace:hasBitstream rdf:resource=""/> <dc:contributor>Nymark, Marianne</dc:contributor> <dcterms:title>Genome editing in diatoms : achievements and goals</dcterms:title> <dc:creator>Jaubert, Marianne</dc:creator> <dc:creator>Ferrante, Maria I.</dc:creator> <dcterms:isPartOf rdf:resource=""/> <dcterms:available rdf:datatype="">2019-01-16T09:16:55Z</dcterms:available> <dc:contributor>Jaubert, Marianne</dc:contributor> <dc:creator>Kolot, Misha</dc:creator> <dc:date rdf:datatype="">2019-01-16T09:16:55Z</dc:date> <dspace:isPartOfCollection rdf:resource=""/> <dc:contributor>Rio Bartulos, Carolina</dc:contributor> <dc:creator>Rio Bartulos, Carolina</dc:creator> <dc:creator>Falciatore, Angela</dc:creator> <dc:contributor>Daboussi, Fayza</dc:contributor> <dc:contributor>Ferrante, Maria I.</dc:contributor> <dc:creator>Serif, Manuel</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Bones, Atle M.</dc:creator> <dcterms:rights rdf:resource=""/> </rdf:Description> </rdf:RDF>

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