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Melting Transition of Oriented DNA Fibers Submerged in Poly(ethylene glycol) Solutions Studied by Neutron Scattering and Calorimetry

Melting Transition of Oriented DNA Fibers Submerged in Poly(ethylene glycol) Solutions Studied by Neutron Scattering and Calorimetry

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GONZÁLEZ, Adrián, Andrew WILDES, Marta MARTY-RODA, Santiago CUESTA-LÓPEZ, Estelle MOSSOU, Andrew STUDER, Bruno DEMÉ, Gaël MOIROUX, Jean-Luc GARDEN, Nikos THEODORAKOPOULOS, 2018. Melting Transition of Oriented DNA Fibers Submerged in Poly(ethylene glycol) Solutions Studied by Neutron Scattering and Calorimetry. In: The Journal of Physical Chemistry B. 122(9), pp. 2504-2515. ISSN 1520-6106. eISSN 1520-5207. Available under: doi: 10.1021/acs.jpcb.7b11608

@article{Gonzalez2018-03-08Melti-43506, title={Melting Transition of Oriented DNA Fibers Submerged in Poly(ethylene glycol) Solutions Studied by Neutron Scattering and Calorimetry}, year={2018}, doi={10.1021/acs.jpcb.7b11608}, number={9}, volume={122}, issn={1520-6106}, journal={The Journal of Physical Chemistry B}, pages={2504--2515}, author={González, Adrián and Wildes, Andrew and Marty-Roda, Marta and Cuesta-López, Santiago and Mossou, Estelle and Studer, Andrew and Demé, Bruno and Moiroux, Gaël and Garden, Jean-Luc and Theodorakopoulos, Nikos} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/43506"> <dc:contributor>González, Adrián</dc:contributor> <dc:contributor>Theodorakopoulos, Nikos</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/41"/> <dc:creator>Wildes, Andrew</dc:creator> <dc:creator>Cuesta-López, Santiago</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Studer, Andrew</dc:creator> <dc:contributor>Studer, Andrew</dc:contributor> <dc:creator>González, Adrián</dc:creator> <dc:contributor>Garden, Jean-Luc</dc:contributor> <dc:creator>Theodorakopoulos, Nikos</dc:creator> <dc:contributor>Mossou, Estelle</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-10-12T09:06:22Z</dc:date> <dcterms:issued>2018-03-08</dcterms:issued> <dcterms:title>Melting Transition of Oriented DNA Fibers Submerged in Poly(ethylene glycol) Solutions Studied by Neutron Scattering and Calorimetry</dcterms:title> <dc:creator>Demé, Bruno</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Marty-Roda, Marta</dc:creator> <dc:creator>Moiroux, Gaël</dc:creator> <dc:contributor>Wildes, Andrew</dc:contributor> <dc:language>eng</dc:language> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/43506"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/41"/> <dc:creator>Mossou, Estelle</dc:creator> <dcterms:abstract xml:lang="eng">The influence of molecular confinement on the melting transition of oriented Na-DNA fibers submerged in poly(ethylene glycol) (PEG) solutions has been studied. The PEG solution exerts an osmotic pressure on the fibers which, in turn, is related to the DNA intermolecular distance. Calorimetry measurements show that the melting temperature increases and the width of the transition decreases with decreasing intermolecular distance. Neutron scattering was used to monitor the integrated intensity and width of a Bragg peak from the B-form of DNA as a function of temperature. The data were quantitatively analyzed using the Peyrard–Bishop–Dauxois model. The experiments and analysis showed that long segments of double-stranded DNA persist until the last stages of melting and that there appears to be a substantial increase of the DNA dynamics as the melting temperature of the DNA is approached.</dcterms:abstract> <dc:contributor>Demé, Bruno</dc:contributor> <dc:creator>Garden, Jean-Luc</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-10-12T09:06:22Z</dcterms:available> <dc:contributor>Cuesta-López, Santiago</dc:contributor> <dc:contributor>Marty-Roda, Marta</dc:contributor> <dc:contributor>Moiroux, Gaël</dc:contributor> </rdf:Description> </rdf:RDF>

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