KOPS - The Institutional Repository of the University of Konstanz

Distinct Mechanisms Regulate Lck Spatial Organization in Activated T Cells

Distinct Mechanisms Regulate Lck Spatial Organization in Activated T Cells

Cite This

Files in this item

Checksum: MD5:8cfaedc7d82afaddd9c2c0a8dd5560b9

KAPOOR-KAUSHIK, Natasha, Elizabeth HINDE, Ewoud B. COMPEER, Yui YAMAMOTO, Felix KRAUS, Zhengmin YANG, Jieqiong LOU, Sophie V. PAGEON, Katharina GAUS, Jérémie ROSSY, 2016. Distinct Mechanisms Regulate Lck Spatial Organization in Activated T Cells. In: Frontiers in Immunology. 7, 83. eISSN 1664-3224. Available under: doi: 10.3389/fimmu.2016.00083

@article{KapoorKaushik2016Disti-42995, title={Distinct Mechanisms Regulate Lck Spatial Organization in Activated T Cells}, year={2016}, doi={10.3389/fimmu.2016.00083}, volume={7}, journal={Frontiers in Immunology}, author={Kapoor-Kaushik, Natasha and Hinde, Elizabeth and Compeer, Ewoud B. and Yamamoto, Yui and Kraus, Felix and Yang, Zhengmin and Lou, Jieqiong and Pageon, Sophie V. and Gaus, Katharina and Rossy, Jérémie}, note={Article Number: 83} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/42995"> <dc:creator>Kapoor-Kaushik, Natasha</dc:creator> <dc:contributor>Compeer, Ewoud B.</dc:contributor> <dc:creator>Kraus, Felix</dc:creator> <dc:contributor>Yamamoto, Yui</dc:contributor> <dc:creator>Hinde, Elizabeth</dc:creator> <dcterms:abstract xml:lang="eng">Phosphorylation of the T cell receptor (TCR) by the kinase Lck is the first detectable signaling event upon antigen engagement. The distribution of Lck within the plasma membrane, its conformational state, kinase activity, and protein-protein interactions all contribute to determine how efficiently Lck phosphorylates the engaged TCR. Here, we used cross-correlation raster image correlation spectroscopy and photoactivated localization microscopy to identify two mechanisms of Lck clustering: an intrinsic mechanism of Lck clustering induced by locking Lck in its open conformation and an extrinsic mechanism of clustering controlled by the phosphorylation of tyrosine 192, which regulates the affinity of Lck SH2 domain. Both mechanisms of clustering were differently affected by the absence of the kinase Zap70 or the adaptor Lat. We further observed that the adaptor TSAd bound to and promoted the diffusion of Lck when it is phosphorylated on tyrosine 192. Our data suggest that while Lck open conformation drives aggregation and clustering, the spatial organization of Lck is further controlled by signaling events downstream of TCR phosphorylation.</dcterms:abstract> <dc:language>eng</dc:language> <dc:creator>Lou, Jieqiong</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/42995/1/Kapoor-Kaushik_2-1nz7jqirnd2gx5.pdf"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-08-07T12:03:08Z</dcterms:available> <dc:creator>Gaus, Katharina</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:rights>Attribution 4.0 International</dc:rights> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-08-07T12:03:08Z</dc:date> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Yamamoto, Yui</dc:creator> <dc:contributor>Pageon, Sophie V.</dc:contributor> <dc:contributor>Kapoor-Kaushik, Natasha</dc:contributor> <dc:contributor>Hinde, Elizabeth</dc:contributor> <dc:contributor>Rossy, Jérémie</dc:contributor> <dc:contributor>Kraus, Felix</dc:contributor> <dc:contributor>Gaus, Katharina</dc:contributor> <dc:creator>Pageon, Sophie V.</dc:creator> <dc:creator>Rossy, Jérémie</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/42995/1/Kapoor-Kaushik_2-1nz7jqirnd2gx5.pdf"/> <dcterms:issued>2016</dcterms:issued> <dc:contributor>Lou, Jieqiong</dc:contributor> <dc:creator>Compeer, Ewoud B.</dc:creator> <dc:creator>Yang, Zhengmin</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42995"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:title>Distinct Mechanisms Regulate Lck Spatial Organization in Activated T Cells</dcterms:title> <dc:contributor>Yang, Zhengmin</dc:contributor> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> </rdf:Description> </rdf:RDF>

Downloads since Aug 7, 2018 (Information about access statistics)

Kapoor-Kaushik_2-1nz7jqirnd2gx5.pdf 232

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


Browse

My Account