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Transient DNA Damage Following Exposure to Gold Nanoparticles

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MAY, Sarah, Cordula HIRSCH, Alexandra RIPPL, Nils BOHMER, Jean-Pierre KAISER, Liliane DIENER, Adrian WICHSER, Alexander BÜRKLE, Peter WICK, 2018. Transient DNA Damage Following Exposure to Gold Nanoparticles. In: Nanoscale. 10(33), pp. 15723-15735. ISSN 2040-3364. eISSN 2040-3372. Available under: doi: 10.1039/C8NR03612H

@article{May2018Trans-42962, title={Transient DNA Damage Following Exposure to Gold Nanoparticles}, year={2018}, doi={10.1039/C8NR03612H}, number={33}, volume={10}, issn={2040-3364}, journal={Nanoscale}, pages={15723--15735}, author={May, Sarah and Hirsch, Cordula and Rippl, Alexandra and Bohmer, Nils and Kaiser, Jean-Pierre and Diener, Liliane and Wichser, Adrian and Bürkle, Alexander and Wick, Peter} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/42962"> <dc:creator>Diener, Liliane</dc:creator> <dc:creator>Wick, Peter</dc:creator> <dc:creator>Rippl, Alexandra</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/42962/1/May_2-1ggyvw19bj1c44.pdf"/> <dc:contributor>Rippl, Alexandra</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dc:creator>Kaiser, Jean-Pierre</dc:creator> <dc:creator>Bürkle, Alexander</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42962"/> <dc:contributor>Bürkle, Alexander</dc:contributor> <dc:contributor>Hirsch, Cordula</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Bohmer, Nils</dc:creator> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Hirsch, Cordula</dc:creator> <dc:contributor>Wick, Peter</dc:contributor> <dc:contributor>Wichser, Adrian</dc:contributor> <dcterms:abstract xml:lang="eng">Due to their interesting physicochemical properties, gold nanoparticles (Au-NPs) are the focus of increasing attention in the field of biomedicine and are under consideration for use in drug delivery and bioimaging, or as radiosensitizers and nano-based vaccines. Thorough evaluation of the genotoxic potential of Au-NPs is required, since damage to the genome can remain undetected in standard hazard assessments. Available genotoxicity data is either limited or contradictory. Here, we examined the influence of three surface modified 3–4 nm Au-NPs on human A549 cells, according to the reactive oxygen species (ROS) paradigm. After 24 h of Au-NP treatment, nanoparticles were taken up by cells as agglomerates; however, no influence on cell viability or inflammation was detected. No increase in ROS production was observed by H2- DCF assay; however, intracellular glutathione levels reduced over time, indicating oxidative stress. All three types of Au- NPs induced DNA damage, as detected by alkaline comet assay. The strongest genotoxic effect was observed for positively charged Au-NP I. Further analysis of Au-NP I by neutral comet assay, fluorimetric detection of alkaline DNA unwinding assay, and γH2AX staining, revealed that the induced DNA lesions were predominantly at alkali-labile sites. As highly controlled repair mechanisms have evolved to remove a wide range of DNA lesions with great efficiency, it is important to focus on both acute cyto- and genotoxicity, alongside post-treatment effects and DNA repair. We demonstrate that Au-NPinduced DNA damage is largely repaired over time, indicating that the observed damage is of transient nature.</dcterms:abstract> <dc:creator>May, Sarah</dc:creator> <dc:contributor>Bohmer, Nils</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-08-03T11:34:00Z</dc:date> <dc:contributor>May, Sarah</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-08-03T11:34:00Z</dcterms:available> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:language>eng</dc:language> <dc:contributor>Kaiser, Jean-Pierre</dc:contributor> <dc:creator>Wichser, Adrian</dc:creator> <dcterms:issued>2018</dcterms:issued> <dcterms:title>Transient DNA Damage Following Exposure to Gold Nanoparticles</dcterms:title> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/42962/1/May_2-1ggyvw19bj1c44.pdf"/> <dc:contributor>Diener, Liliane</dc:contributor> </rdf:Description> </rdf:RDF>

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