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Rpn7 Is Required for the Structural Integrity of the 26 S Proteasome of Saccharomyces cerevisiae

Rpn7 Is Required for the Structural Integrity of the 26 S Proteasome of Saccharomyces cerevisiae

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ISONO, Erika, Yasushi SAEKI, Hideyoshi YOKOSAWA, Akio TOH-E, 2004. Rpn7 Is Required for the Structural Integrity of the 26 S Proteasome of Saccharomyces cerevisiae. In: The Journal of Biological Chemistry : JBC. 279(26), pp. 27168-27176. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M314231200

@article{Isono2004-06-25Requi-42151, title={Rpn7 Is Required for the Structural Integrity of the 26 S Proteasome of Saccharomyces cerevisiae}, year={2004}, doi={10.1074/jbc.M314231200}, number={26}, volume={279}, issn={0021-9258}, journal={The Journal of Biological Chemistry : JBC}, pages={27168--27176}, author={Isono, Erika and Saeki, Yasushi and Yokosawa, Hideyoshi and Toh-e, Akio} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/42151"> <dcterms:title>Rpn7 Is Required for the Structural Integrity of the 26 S Proteasome of Saccharomyces cerevisiae</dcterms:title> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-04-24T12:46:59Z</dc:date> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-04-24T12:46:59Z</dcterms:available> <dc:contributor>Toh-e, Akio</dc:contributor> <dcterms:issued>2004-06-25</dcterms:issued> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Isono, Erika</dc:creator> <dc:creator>Saeki, Yasushi</dc:creator> <dc:contributor>Isono, Erika</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Yokosawa, Hideyoshi</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Saeki, Yasushi</dc:contributor> <dc:contributor>Yokosawa, Hideyoshi</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42151"/> <dc:creator>Toh-e, Akio</dc:creator> <dcterms:abstract xml:lang="eng">Rpn7 is one of the lid subunits of the 26 S proteasome regulatory particle. The RPN7 gene is known to be essential, but its function remains to be elucidated. To explore the function of Rpn7, we isolated and characterized temperature-sensitive rpn7 mutants. All of the rpn7 mutants obtained accumulated poly-ubiquitinated proteins when grown at the restrictive temperature. The N-end rule substrate (Ub-Arg-beta-galactosidase), the UFD pathway substrate (Ub-Pro-beta-galactosidase), and cell cycle regulators (Pds1 and Clb2) were found to be stabilized in experiments using one of the rpn7 mutants termed rpn7-3 at the restrictive temperature, indicating its defect in the ubiquitin-proteasome pathway. Subsequent analysis of the structure of the 26 S proteasome in rpn7-3 cells suggested that the defect was in the assembly of the 26 S holoenzyme. The most striking characteristic of the proteasome of the rpn7-3 mutant was that a lid subcomplex affinity-purified from the rpn7-3 cells grown at the restrictive temperature contained only 5 of the 8 lid components, a phenomenon that has not been reported in the previously isolated lid mutants. From these results, we concluded that Rpn7 is required for the integrity of the 26 S complex by establishing a correct lid structure.</dcterms:abstract> </rdf:Description> </rdf:RDF>

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