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The expression profile of the ubiquitin-like modifier FAT10 in immune cells suggests cell type-specific functions

The expression profile of the ubiquitin-like modifier FAT10 in immune cells suggests cell type-specific functions

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SCHREGLE, Richard, Mei Min MAH, Stefanie MUELLER, Annette AICHEM, Michael BASLER, Marcus GROETTRUP, 2018. The expression profile of the ubiquitin-like modifier FAT10 in immune cells suggests cell type-specific functions. In: Immunogenetics. 70(7), pp. 429-438. ISSN 0093-7711. eISSN 1432-1211. Available under: doi: 10.1007/s00251-018-1055-5

@article{Schregle2018-07expre-41974, title={The expression profile of the ubiquitin-like modifier FAT10 in immune cells suggests cell type-specific functions}, year={2018}, doi={10.1007/s00251-018-1055-5}, number={7}, volume={70}, issn={0093-7711}, journal={Immunogenetics}, pages={429--438}, author={Schregle, Richard and Mah, Mei Min and Mueller, Stefanie and Aichem, Annette and Basler, Michael and Groettrup, Marcus} }

Groettrup, Marcus Mah, Mei Min Mueller, Stefanie terms-of-use Basler, Michael 2018-04-09T13:28:36Z The expression profile of the ubiquitin-like modifier FAT10 in immune cells suggests cell type-specific functions Basler, Michael Mueller, Stefanie The TNF and IFN-γ-inducible ubiquitin-like modifier HLA-F adjacent transcript 10 (FAT10) is most prominently expressed in immunological tissues but information regarding basal expression and inducibility of FAT10 in the different types of immune cells is still lacking. Hence, we investigated FAT10 mRNA expression in the major human and murine immune cell subsets, and FAT10 protein expression in human leukocytes. We isolated the different human leukocytes from peripheral blood and the murine immune cell subsets from spleen. The purified leukocytes were left untreated or stimulated with TNF and INF-γ or LPS to induce FAT10 followed by quantitative real-time PCR or western blot analysis. Basal expression of FAT10 mRNA and protein was generally low but strongly up-regulated by IFN-γ and TNF in all immune cell subsets. LPS treatment induced FAT10 expression marginally in human CD8<sup>+</sup> T cells and murine granulocytes, but it increased Fat10 expression significantly in murine regulatory T cells. Yet, in human CD8<sup>+</sup> T cells, natural killer cells, natural killer T cells, and dendritic cells, the FAT10 mRNA was expressed without induction. Similarly, murine macrophages, monocytes, and regulatory T cells expressed Fat10 in the absence of stimulation. In summary, our findings suggest particular functions of FAT10 in these cell types. Furthermore, we observed not only a cell type-specific but also a species-specific basal FAT10 expression profile. Our data will serve as a guideline for future investigations to further elucidate FAT10’s role in the immune system. Groettrup, Marcus Aichem, Annette 2018-07 Mah, Mei Min Aichem, Annette 2018-04-09T13:28:36Z eng Schregle, Richard Schregle, Richard

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