Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können kommenden Montag und Dienstag keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted next Monday and Tuesday.)
Type of Publication: | Journal article |
Publication status: | Published |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-2-174wr1gigkuyo6 |
Author: | Felletti, Michele; Bieber, Anna; Hartig, Jörg S. |
Year of publication: | 2017 |
Published in: | RNA Biology ; 14 (2017), 11. - pp. 1522-1533. - ISSN 1547-6286. - eISSN 1555-8584 |
DOI (citable link): | https://dx.doi.org/10.1080/15476286.2016.1240141 |
Summary: |
Besides its primary informational role, the sequence of the mRNA (mRNA) including its 5′- and 3′- untranslated regions (UTRs), contains important features that are relevant for post-transcriptional and translational regulation of gene expression. In this work a number of bacterial twister motifs are characterized both in vitro and in vivo. The analysis of their genetic contexts shows that these motifs have the potential of being transcribed as part of polycistronic mRNAs, thus we suggest the involvement of bacterial twister motifs in the processing of mRNA. Our data show that the ribozyme-mediated cleavage of the bacterial 3′-UTR has major effects on gene expression. While the observed effects correlate weakly with the kinetic parameters of the ribozymes, they show dependence on motif-specific structural features and on mRNA stabilization properties of the secondary structures that remain on the 3′-UTR after ribozyme cleavage. Using these principles, novel artificial twister-based riboswitches are developed that exert their activity via ligand-dependent cleavage of the 3′-UTR and the removal of the protective intrinsic terminator. Our results provide insights into possible biological functions of these recently discovered and widespread catalytic RNA motifs and offer new tools for applications in biotechnology, synthetic biology and metabolic engineering.
|
Subject (DDC): | 540 Chemistry |
Keywords: | Aptazyme, bacteria, hammerhead ribozyme, polyadenylation, riboswitch, RNase, RNA decay, secondary structure, twister ribozyme |
Link to License: | In Copyright |
Bibliography of Konstanz: | Yes |
FELLETTI, Michele, Anna BIEBER, Jörg S. HARTIG, 2017. The 3′-untranslated region of mRNAs as a site for ribozyme cleavage-dependent processing and control in bacteria. In: RNA Biology. 14(11), pp. 1522-1533. ISSN 1547-6286. eISSN 1555-8584. Available under: doi: 10.1080/15476286.2016.1240141
@article{Felletti20173untr-41533, title={The 3′-untranslated region of mRNAs as a site for ribozyme cleavage-dependent processing and control in bacteria}, year={2017}, doi={10.1080/15476286.2016.1240141}, number={11}, volume={14}, issn={1547-6286}, journal={RNA Biology}, pages={1522--1533}, author={Felletti, Michele and Bieber, Anna and Hartig, Jörg S.} }
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/41533"> <dc:contributor>Felletti, Michele</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Bieber, Anna</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/41533/1/Felletti_2-174wr1gigkuyo6.pdf"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-02-21T10:43:42Z</dcterms:available> <dcterms:title>The 3′-untranslated region of mRNAs as a site for ribozyme cleavage-dependent processing and control in bacteria</dcterms:title> <dcterms:issued>2017</dcterms:issued> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/41533"/> <dc:creator>Hartig, Jörg S.</dc:creator> <dcterms:abstract xml:lang="eng">Besides its primary informational role, the sequence of the mRNA (mRNA) including its 5′- and 3′- untranslated regions (UTRs), contains important features that are relevant for post-transcriptional and translational regulation of gene expression. In this work a number of bacterial twister motifs are characterized both in vitro and in vivo. The analysis of their genetic contexts shows that these motifs have the potential of being transcribed as part of polycistronic mRNAs, thus we suggest the involvement of bacterial twister motifs in the processing of mRNA. Our data show that the ribozyme-mediated cleavage of the bacterial 3′-UTR has major effects on gene expression. While the observed effects correlate weakly with the kinetic parameters of the ribozymes, they show dependence on motif-specific structural features and on mRNA stabilization properties of the secondary structures that remain on the 3′-UTR after ribozyme cleavage. Using these principles, novel artificial twister-based riboswitches are developed that exert their activity via ligand-dependent cleavage of the 3′-UTR and the removal of the protective intrinsic terminator. Our results provide insights into possible biological functions of these recently discovered and widespread catalytic RNA motifs and offer new tools for applications in biotechnology, synthetic biology and metabolic engineering.</dcterms:abstract> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dc:contributor>Hartig, Jörg S.</dc:contributor> <dc:creator>Felletti, Michele</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-02-21T10:43:42Z</dc:date> <dc:rights>terms-of-use</dc:rights> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/41533/1/Felletti_2-174wr1gigkuyo6.pdf"/> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:language>eng</dc:language> <dc:creator>Bieber, Anna</dc:creator> </rdf:Description> </rdf:RDF>
Felletti_2-174wr1gigkuyo6.pdf | 193 |