## Identification of sVSG117 as an Immunodiagnostic Antigen and Evaluation of a Dual-Antigen Lateral Flow Test for the Diagnosis of Human African Trypanosomiasis

2014
##### Authors
Sullivan, Lauren
Sastry, Lalitha
Mehlert, Angela
Wall, Steven J.
Ferguson, Michael A. J.
Journal article
Published
##### Published in
PLoS Neglected Tropical Diseases ; 8 (2014), 7. - e2976. - ISSN 1935-2727. - eISSN 1935-2735
##### Abstract
Human African Trypanosomiasis (HAT) is caused by infection with Trypanosoma brucei gambiense or T. b. rhodesiense. The diagnosis of T. b. gambiense infections currently relies primarily on a Card Agglutination Test for Trypanosomiasis (CATT), which has acknowledged limitations, and there is no simple test for T. b. rhodesiense infection. Our overall aim is to produce a simple lateral flow test device with a similar or better sensitivity and specificity than CATT but with better stability and ease of use at point of care. In this study, we identified a particular variant surface glycoprotein, sVSG117, with good diagnostic potential and combined it with a previously identified recombinant diagnostic antigen, rISG65, to produce a prototype dual-antigen lateral flow test. We performed a virtual field trial by testing the device blind with 431 randomized serum samples provided by the WHO HAT Specimen Biobank. The results show that, although the prototype lateral flow test is un-optimized, it was able to diagnose T. b. gambiense HAT with a sensitivity and specificity of 97.3% and 83.3% and T. b. rhodesiense HAT with a sensitivity and specificity of 83.9% and 85.3%.
##### Subject (DDC)
570 Biosciences, Biology
##### Cite This
ISO 690SULLIVAN, Lauren, Jennifer S. FLEMING, Lalitha SASTRY, Angela MEHLERT, Steven J. WALL, Michael A. J. FERGUSON, 2014. Identification of sVSG117 as an Immunodiagnostic Antigen and Evaluation of a Dual-Antigen Lateral Flow Test for the Diagnosis of Human African Trypanosomiasis. In: PLoS Neglected Tropical Diseases. 8(7), e2976. ISSN 1935-2727. eISSN 1935-2735. Available under: doi: 10.1371/journal.pntd.0002976
BibTex
@article{Sullivan2014-07-17Ident-41305,
year={2014},
doi={10.1371/journal.pntd.0002976},
title={Identification of sVSG117 as an Immunodiagnostic Antigen and Evaluation of a Dual-Antigen Lateral Flow Test for the Diagnosis of Human African Trypanosomiasis},
number={7},
volume={8},
issn={1935-2727},
journal={PLoS Neglected Tropical Diseases},
author={Sullivan, Lauren and Fleming, Jennifer S. and Sastry, Lalitha and Mehlert, Angela and Wall, Steven J. and Ferguson, Michael A. J.},
note={Article Number: e2976}
}

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