KOPS - The Institutional Repository of the University of Konstanz

RPTEC/TERT1 cells form highly differentiated tubules when cultured in a 3D matrix

Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können kommenden Montag und Dienstag keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted next Monday and Tuesday.)

RPTEC/TERT1 cells form highly differentiated tubules when cultured in a 3D matrix

Cite This

Files in this item

Checksum: MD5:fef116f67764390b3d29a89219c869f8

SECKER, Philipp F., Lisanne LUKS, Nadja SCHLICHENMAIER, Daniel R. DIETRICH, 2018. RPTEC/TERT1 cells form highly differentiated tubules when cultured in a 3D matrix. In: Alternatives to Animal Experimentation : ALTEX. 35(2), pp. 223-234. ISSN 1868-596X. eISSN 1868-8551. Available under: doi: 10.14573/altex.1710181

@article{Secker2018RPTEC-41028, title={RPTEC/TERT1 cells form highly differentiated tubules when cultured in a 3D matrix}, year={2018}, doi={10.14573/altex.1710181}, number={2}, volume={35}, issn={1868-596X}, journal={Alternatives to Animal Experimentation : ALTEX}, pages={223--234}, author={Secker, Philipp F. and Luks, Lisanne and Schlichenmaier, Nadja and Dietrich, Daniel R.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/41028"> <dc:contributor>Dietrich, Daniel R.</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-01-09T15:45:13Z</dcterms:available> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/41028/1/Secker_2-3mnb1gl0qpx23.pdf"/> <dc:creator>Dietrich, Daniel R.</dc:creator> <dc:creator>Schlichenmaier, Nadja</dc:creator> <dc:rights>Attribution 4.0 International</dc:rights> <dc:contributor>Schlichenmaier, Nadja</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-01-09T15:45:13Z</dc:date> <dc:language>eng</dc:language> <dc:creator>Secker, Philipp F.</dc:creator> <dcterms:abstract xml:lang="eng">The proximal tubule is the primary site for renal solute reabsorption and secretion and thus a main target for drug-induced toxicity. Current nonclinical methods using 2D cell cultures are unable to fully recapitulate clinical drug responses mainly due to limited in vitro functional lifespan. Since extracellular matrices are known to be key regulators of cell development, culturing cells on classic 2D plastic surfaces inevitably results in loss of differentiation. Hence, 3D models of the human proximal tubule that recapitulate the in vivo morphology would allow for improved drug screening and disease modeling. Here, the development and characterization of a 3D proximal tubule model using RPTEC/TERT1 cells is presented. RPTEC/TERT1 cells self-assembled in matrigel to form highly differentiated and stable 3D tubular structures characterized by a branched network of monolayered cells encircling a cell-free lumen thus mimicking the proximal tubule. In vitro tubuli resembled the polarity of a proximal tubule epithelium as indicated by polar expression of Na+/K+- ATPase and ZO-3. Furthermore, 3D cultured RPTEC/TERT1 cells showed overall increased mRNA expression of xenobiotic transporters e.g. OCTs and MATEs and de novo expression of OAT3 when compared to cultures on plastics or membrane inserts. Finally, this model was used to assess delayed cisplatin-induced nephrotoxicity and demonstrated increased sensitivity when compared to 2D culture. Thus, the easy-to-use model described here may prove to be useful for mechanistic investigations, e.g. in discovery of compounds interfering with tubule formation, differentiation and polarization, as well for the detection and understanding of pharmaceutical induced nephrotoxicity.</dcterms:abstract> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dcterms:title>RPTEC/TERT1 cells form highly differentiated tubules when cultured in a 3D matrix</dcterms:title> <dc:contributor>Secker, Philipp F.</dc:contributor> <dc:contributor>Luks, Lisanne</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/41028/1/Secker_2-3mnb1gl0qpx23.pdf"/> <dc:creator>Luks, Lisanne</dc:creator> <dcterms:issued>2018</dcterms:issued> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/41028"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> </rdf:Description> </rdf:RDF>

Downloads since Jan 9, 2018 (Information about access statistics)

Secker_2-3mnb1gl0qpx23.pdf 429

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


My Account