Lessons from GNE-deficient embryonic stem cells : sialic acid biosynthesis is involved in proliferation and gene expression
| Type of Publication: | Journal article |
| Publication status: | Published |
| Author: | Weidemann, Wenke; Klukas, Christian; Klein, Andreas; Simm, Andreas; Schreiber, Falk; Horstkorte, Rüdiger |
| Year of publication: | 2010 |
| Published in: | Glycobiology ; 20 (2010), 1. - pp. 107-117. - ISSN 0959-6658. - eISSN 1460-2423 |
| Pubmed ID: | 19797319 |
| DOI (citable link): | https://dx.doi.org/10.1093/glycob/cwp153 |
| Summary: |
Sialic acids are widely expressed as terminal carbohydrates on glycoconjugates of eukaryotic cells. They are involved in a variety of cellular functions, such as cell adhesion or signal recognition. The key enzyme of sialic acid biosynthesis is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE), which catalyzes the first two steps of sialic acid biosynthesis in the cytosol. Inactivation of GNE causes early embryonic lethality. In this study, we analyzed wild-type and GNE-deficient embryonic stem cells from mice. We found for the first time that proliferation is directly correlated with GNE-expression and the cellular sialic acid concentration. Furthermore, we identified growth-related genes that are differentially expressed in GNE-deficient embryonic stem cells compared to wild-type embryonic stem cells.
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| Subject (DDC): | 004 Computer Science |
| Keywords: | cell differentiation, N -acetylmannosamine , N -acetylmannosamine kinase , UDP- N -acetylglucosamine-2- epimerase |
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WEIDEMANN, Wenke, Christian KLUKAS, Andreas KLEIN, Andreas SIMM, Falk SCHREIBER, Rüdiger HORSTKORTE, 2010. Lessons from GNE-deficient embryonic stem cells : sialic acid biosynthesis is involved in proliferation and gene expression. In: Glycobiology. 20(1), pp. 107-117. ISSN 0959-6658. eISSN 1460-2423. Available under: doi: 10.1093/glycob/cwp153
@article{Weidemann2010Lesso-40405, title={Lessons from GNE-deficient embryonic stem cells : sialic acid biosynthesis is involved in proliferation and gene expression}, year={2010}, doi={10.1093/glycob/cwp153}, number={1}, volume={20}, issn={0959-6658}, journal={Glycobiology}, pages={107--117}, author={Weidemann, Wenke and Klukas, Christian and Klein, Andreas and Simm, Andreas and Schreiber, Falk and Horstkorte, Rüdiger} }
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