Emerging metrology for high-throughput nanomaterial genotoxicology


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NELSON, Bryant C., Christa W. WRIGHT, Yuko IBUKI, Maria MORENO-VILLANUEVA, Hanna L. KARLSSON, Giel HENDRIKS, Christopher M. SIMS, Neenu SINGH, Shareen H. DOAK, 2017. Emerging metrology for high-throughput nanomaterial genotoxicology. In: Mutagenesis. 32(1), pp. 215-232. ISSN 0267-8357. eISSN 1464-3804

@article{Nelson2017Emerg-38843, title={Emerging metrology for high-throughput nanomaterial genotoxicology}, year={2017}, doi={10.1093/mutage/gew037}, number={1}, volume={32}, issn={0267-8357}, journal={Mutagenesis}, pages={215--232}, author={Nelson, Bryant C. and Wright, Christa W. and Ibuki, Yuko and Moreno-Villanueva, Maria and Karlsson, Hanna L. and Hendriks, Giel and Sims, Christopher M. and Singh, Neenu and Doak, Shareen H.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/38843"> <dc:creator>Karlsson, Hanna L.</dc:creator> <dc:creator>Sims, Christopher M.</dc:creator> <dc:contributor>Wright, Christa W.</dc:contributor> <dc:creator>Nelson, Bryant C.</dc:creator> <dc:creator>Singh, Neenu</dc:creator> <dc:contributor>Karlsson, Hanna L.</dc:contributor> <dc:contributor>Moreno-Villanueva, Maria</dc:contributor> <dc:contributor>Hendriks, Giel</dc:contributor> <dcterms:title>Emerging metrology for high-throughput nanomaterial genotoxicology</dcterms:title> <dc:creator>Doak, Shareen H.</dc:creator> <dc:creator>Moreno-Villanueva, Maria</dc:creator> <dc:contributor>Doak, Shareen H.</dc:contributor> <dc:creator>Wright, Christa W.</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dc:contributor>Singh, Neenu</dc:contributor> <dc:contributor>Ibuki, Yuko</dc:contributor> <dcterms:issued>2017</dcterms:issued> <dc:creator>Hendriks, Giel</dc:creator> <dc:contributor>Sims, Christopher M.</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-11T09:56:57Z</dc:date> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-05-11T09:56:57Z</dcterms:available> <dcterms:abstract xml:lang="eng">The rapid development of the engineered nanomaterial (ENM) manufacturing industry has accelerated the incorporation of ENMs into a wide variety of consumer products across the globe. Unintentionally or not, some of these ENMs may be introduced into the environment or come into contact with humans or other organisms resulting in unexpected biological effects. It is thus prudent to have rapid and robust analytical metrology in place that can be used to critically assess and/or predict the cytotoxicity, as well as the potential genotoxicity of these ENMs. Many of the traditional genotoxicity test methods [e.g. unscheduled DNA synthesis assay, bacterial reverse mutation (Ames) test, etc.,] for determining the DNA damaging potential of chemical and biological compounds are not suitable for the evaluation of ENMs, due to a variety of methodological issues ranging from potential assay interferences to problems centered on low sample throughput. Recently, a number of sensitive, high-throughput genotoxicity assays/platforms (CometChip assay, flow cytometry/micronucleus assay, flow cytometry/γ-H2AX assay, automated 'Fluorimetric Detection of Alkaline DNA Unwinding' (FADU) assay, ToxTracker reporter assay) have been developed, based on substantial modifications and enhancements of traditional genotoxicity assays. These new assays have been used for the rapid measurement of DNA damage (strand breaks), chromosomal damage (micronuclei) and for detecting upregulated DNA damage signalling pathways resulting from ENM exposures. In this critical review, we describe and discuss the fundamental measurement principles and measurement endpoints of these new assays, as well as the modes of operation, analytical metrics and potential interferences, as applicable to ENM exposures. An unbiased discussion of the major technical advantages and limitations of each assay for evaluating and predicting the genotoxic potential of ENMs is also provided.</dcterms:abstract> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38843"/> <dc:creator>Ibuki, Yuko</dc:creator> <dc:contributor>Nelson, Bryant C.</dc:contributor> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 11.05.2017 (Informationen über die Zugriffsstatistik)

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