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Assembly of a protein “brush” by end-grafting titin fragments to liposomes

Assembly of a protein “brush” by end-grafting titin fragments to liposomes

Type of Publication: Journal article
Publication status: Published
Author: Chami, Mohamed; Franke, Barbara; Mayans, Olga; Kreplak, Laurent
Year of publication: 2011
Published in: Journal of Bioscience and Bioengineering ; 112 (2011), 2. - pp. 178-179. - ISSN 1389-1723. - eISSN 1347-4421
Pubmed ID: 21616710
DOI (citable link): https://dx.doi.org/10.1016/j.jbiosc.2011.04.014
Summary:
Titin is a giant filamentous protein of striated muscle composed of >300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features.
Subject (DDC): 570 Biosciences, Biology
Keywords: Decorated liposomes; Titin; Cryo-electron microscopy; His-Tag; Nickel chelating lipid

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CHAMI, Mohamed, Barbara FRANKE, Olga MAYANS, Laurent KREPLAK, 2011. Assembly of a protein “brush” by end-grafting titin fragments to liposomes. In: Journal of Bioscience and Bioengineering. 112(2), pp. 178-179. ISSN 1389-1723. eISSN 1347-4421. Available under: doi: 10.1016/j.jbiosc.2011.04.014

@article{Chami2011-08Assem-38668, title={Assembly of a protein “brush” by end-grafting titin fragments to liposomes}, year={2011}, doi={10.1016/j.jbiosc.2011.04.014}, number={2}, volume={112}, issn={1389-1723}, journal={Journal of Bioscience and Bioengineering}, pages={178--179}, author={Chami, Mohamed and Franke, Barbara and Mayans, Olga and Kreplak, Laurent} }

Mayans, Olga Chami, Mohamed eng 2017-05-02T08:32:39Z Kreplak, Laurent 2011-08 Franke, Barbara Assembly of a protein “brush” by end-grafting titin fragments to liposomes Franke, Barbara Mayans, Olga 2017-05-02T08:32:39Z Titin is a giant filamentous protein of striated muscle composed of >300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features. Kreplak, Laurent Chami, Mohamed

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