Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling
| Type of Publication: | Contribution to a collection |
| Publication status: | Published |
| Author: | Grafahrend-Belau, Eva; Junker, Astrid; Schreiber, Falk; Junker, Björn H. |
| Year of publication: | 2014 |
| Published in: | Plant Metabolic Flux Analysis / Dieuaide-Noubhani, Martine; Alonso, Ana Paula (ed.). - Totowa, NJ : Humana Press, 2014. - (Methods in Molecular Biology ; 1090). - pp. 281-299. - ISBN 978-1-62703-687-0 |
| DOI (citable link): | https://dx.doi.org/10.1007/978-1-62703-688-7_17 |
| Summary: |
The analysis of plant metabolic networks essentially contributes to the understanding of the efficiency of plant systems in terms of their biotechnological usage. Metabolic fluxes are determined by biochemical parameters such as metabolite concentrations as well as enzyme properties and activities, which in turn are the result of various regulatory events at various levels between control of transcription and posttranslational regulation of enzyme protein activity. Thus, knowledge about metabolic fluxes on a large scale provides an integrated view on the functional state of a metabolically active cell, organ, or system. In this chapter, we introduce flux balance analysis as a constraint-based method for the prediction of optimal metabolic fluxes in a given metabolic network. Furthermore, we provide a step-by-step protocol for metabolic network reconstruction and constraint-based analysis using the COBRA Toolbox.
|
| Subject (DDC): | 004 Computer Science |
| Keywords: | Flux balance analysis, Metabolic network reconstruction, Matlab, COBRA toolbox |
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GRAFAHREND-BELAU, Eva, Astrid JUNKER, Falk SCHREIBER, Björn H. JUNKER, 2014. Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling. In: DIEUAIDE-NOUBHANI, Martine, ed., Ana Paula ALONSO, ed.. Plant Metabolic Flux Analysis. Totowa, NJ:Humana Press, pp. 281-299. ISBN 978-1-62703-687-0. Available under: doi: 10.1007/978-1-62703-688-7_17
@incollection{GrafahrendBelau2014Balan-38590, title={Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling}, year={2014}, doi={10.1007/978-1-62703-688-7_17}, number={1090}, isbn={978-1-62703-687-0}, address={Totowa, NJ}, publisher={Humana Press}, series={Methods in Molecular Biology}, booktitle={Plant Metabolic Flux Analysis}, pages={281--299}, editor={Dieuaide-Noubhani, Martine and Alonso, Ana Paula}, author={Grafahrend-Belau, Eva and Junker, Astrid and Schreiber, Falk and Junker, Björn H.} }
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