KOPS - Das Institutionelle Repositorium der Universität Konstanz

Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides

Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:490a0e0a4b4f0ea999df0e7fd30210e3

HOTTIN, Audrey, Karin BETZ, Kay DIEDERICHS, Andreas MARX, 2017. Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides. In: Chemistry - A European Journal. 23(9), pp. 2109-2118. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.201604515

@article{Hottin2017-02-10Struc-38270, title={Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides}, year={2017}, doi={10.1002/chem.201604515}, number={9}, volume={23}, issn={0947-6539}, journal={Chemistry - A European Journal}, pages={2109--2118}, author={Hottin, Audrey and Betz, Karin and Diederichs, Kay and Marx, Andreas} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/38270"> <dc:creator>Marx, Andreas</dc:creator> <dc:creator>Betz, Karin</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.de/urn:nbn:de:bsz:352-20150914100631302-4485392-8"/> <dcterms:abstract xml:lang="eng">Efficient incorporation of modified nucleotides by DNA polymerases is essential for many cutting-edge biomolecular technologies. The present study compares the acceptance of either alkene- or alkyne-modified nucleotides by KlenTaq DNA polymerase and provides structural insights into how 7-deaza-adenosine and deoxyuridine with attached alkene-modifications are incorporated into the growing DNA strand. Thereby, we identified modified nucleotides that prove to be superior substrates for KlenTaq DNA polymerase compared with their natural analogues. The knowledge can be used to guide future design of functionalized nucleotide building blocks.</dcterms:abstract> <dc:creator>Diederichs, Kay</dc:creator> <dcterms:title>Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides</dcterms:title> <dcterms:issued>2017-02-10</dcterms:issued> <dc:creator>Hottin, Audrey</dc:creator> <dc:contributor>Marx, Andreas</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-04-03T10:51:34Z</dc:date> <dc:language>eng</dc:language> <dc:contributor>Betz, Karin</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38270"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Hottin, Audrey</dc:contributor> <dc:contributor>Diederichs, Kay</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-04-03T10:51:34Z</dcterms:available> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto