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Proteomic profiling of serological responses to Aspergillus fumigatus antigens in patients with invasive aspergillosis

Proteomic profiling of serological responses to Aspergillus fumigatus antigens in patients with invasive aspergillosis

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TEUTSCHBEIN, Janka, Svenja SIMON, Jasmin LOTHER, Jan SPRINGER, Peter HORTSCHANSKY, C. Oliver MORTON, Jürgen LÖFFLER, Hermann EINSELE, Eibhlin CONNEALLY, Thomas R. ROGERS, Reinhard GUTHKE, Axel A. BRAKHAGE, Olaf KNIEMEYER, 2016. Proteomic profiling of serological responses to Aspergillus fumigatus antigens in patients with invasive aspergillosis. In: Journal of Proteome Research. 15(5), pp. 1580-1591. ISSN 1535-3893. eISSN 1535-3907. Available under: doi: 10.1021/acs.jproteome.6b00042

@article{Teutschbein2016-05-06Prote-33787, title={Proteomic profiling of serological responses to Aspergillus fumigatus antigens in patients with invasive aspergillosis}, year={2016}, doi={10.1021/acs.jproteome.6b00042}, number={5}, volume={15}, issn={1535-3893}, journal={Journal of Proteome Research}, pages={1580--1591}, author={Teutschbein, Janka and Simon, Svenja and Lother, Jasmin and Springer, Jan and Hortschansky, Peter and Morton, C. Oliver and Löffler, Jürgen and Einsele, Hermann and Conneally, Eibhlin and Rogers, Thomas R. and Guthke, Reinhard and Brakhage, Axel A. and Kniemeyer, Olaf} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/33787"> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/36"/> <dc:creator>Löffler, Jürgen</dc:creator> <dc:creator>Rogers, Thomas R.</dc:creator> <dc:creator>Teutschbein, Janka</dc:creator> <dc:contributor>Hortschansky, Peter</dc:contributor> <dc:contributor>Simon, Svenja</dc:contributor> <dc:creator>Kniemeyer, Olaf</dc:creator> <dc:creator>Hortschansky, Peter</dc:creator> <dc:creator>Guthke, Reinhard</dc:creator> <dc:contributor>Lother, Jasmin</dc:contributor> <dc:creator>Lother, Jasmin</dc:creator> <dc:creator>Springer, Jan</dc:creator> <dc:contributor>Löffler, Jürgen</dc:contributor> <dcterms:issued>2016-05-06</dcterms:issued> <dc:language>eng</dc:language> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33787"/> <dc:contributor>Teutschbein, Janka</dc:contributor> <dc:contributor>Morton, C. Oliver</dc:contributor> <dc:contributor>Kniemeyer, Olaf</dc:contributor> <dc:contributor>Springer, Jan</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/36"/> <dc:contributor>Einsele, Hermann</dc:contributor> <dc:creator>Brakhage, Axel A.</dc:creator> <dc:creator>Conneally, Eibhlin</dc:creator> <dc:contributor>Brakhage, Axel A.</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-05-03T09:11:31Z</dcterms:available> <dc:creator>Morton, C. Oliver</dc:creator> <dc:contributor>Conneally, Eibhlin</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-05-03T09:11:31Z</dc:date> <dc:contributor>Rogers, Thomas R.</dc:contributor> <dc:creator>Einsele, Hermann</dc:creator> <dc:contributor>Guthke, Reinhard</dc:contributor> <dcterms:abstract xml:lang="eng">Aspergillus fumigatus is the species that most commonly causes the opportunistic infection invasive aspergillosis (IA) in patients being treated for haematological malignancies. Little is known about the A. fumigatus proteins that trigger the production of Aspergillus-specific IgG antibodies during the course of IA. In order to characterise the serological response to A. fumigatus protein antigens mycelial proteins were separated by 2-D gel electrophoresis. The gels were immunoblotted with sera from patients with probable and proven IA, and control patients without IA. We identified 49 different fungal proteins, which gave a positive IgG antibody signal. Most of these antigens play a role in primary metabolism and stress responses. Overall, our analysis identified 18 novel protein antigens from A. fumigatus. To determine whether these antigens can be used as diagnostic or prognostic markers or exhibit a protective activity we employed supervised machine learning with decision trees. We identified two candidates for further analysis, the protein antigens CpcB, and Shm2. Heterologously-produced Shm2 induced a strongly proinflammatory response in human peripheral blood mononuclear cells after in vitro stimulation. In contrast, CpcB did not activate the immune response of PBMCs. These findings could serve as the basis for the development of an immunotherapy of IA.</dcterms:abstract> <dcterms:title>Proteomic profiling of serological responses to Aspergillus fumigatus antigens in patients with invasive aspergillosis</dcterms:title> <dc:creator>Simon, Svenja</dc:creator> </rdf:Description> </rdf:RDF>

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