KOPS - Das Institutionelle Repositorium der Universität Konstanz

β-Lactoglobulin detected in human milk forms noncovalent complexes with maltooligosaccharides as revealed by chip-nanoelectrospray high-resolution tandem mass spectrometry

β-Lactoglobulin detected in human milk forms noncovalent complexes with maltooligosaccharides as revealed by chip-nanoelectrospray high-resolution tandem mass spectrometry

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

CAPITAN, Florina, Adrian C. ROBU, Catalin SCHIOPU, Constantin ILIE, Brian T. CHAIT, Michael PRZYBYLSKI, Alina D. ZAMFIR, 2015. β-Lactoglobulin detected in human milk forms noncovalent complexes with maltooligosaccharides as revealed by chip-nanoelectrospray high-resolution tandem mass spectrometry. In: Amino Acids. 47(11), pp. 2399-2407. ISSN 0939-4451. eISSN 1438-2199

@article{Capitan2015-Lact-33020, title={β-Lactoglobulin detected in human milk forms noncovalent complexes with maltooligosaccharides as revealed by chip-nanoelectrospray high-resolution tandem mass spectrometry}, year={2015}, doi={10.1007/s00726-015-2030-1}, number={11}, volume={47}, issn={0939-4451}, journal={Amino Acids}, pages={2399--2407}, author={Capitan, Florina and Robu, Adrian C. and Schiopu, Catalin and Ilie, Constantin and Chait, Brian T. and Przybylski, Michael and Zamfir, Alina D.} }

Schiopu, Catalin 2015 Chait, Brian T. Ilie, Constantin Capitan, Florina Zamfir, Alina D. 2016-02-18T08:51:30Z Ilie, Constantin Robu, Adrian C. eng Przybylski, Michael Przybylski, Michael β-Lactoglobulin detected in human milk forms noncovalent complexes with maltooligosaccharides as revealed by chip-nanoelectrospray high-resolution tandem mass spectrometry Capitan, Florina Cow's milk protein allergy in exclusively breastfed infants, the main cause of food intolerance during the first 6 months of life, is triggered by the mother's diet. β-Lactoglobulin (BLG) present in cow's milk is one of the most potent allergens for newborns. Since no prophylactic treatment is available, finding ligands capable of binding BLG and reducing its allergenicity is currently the focus of research. In this work, an innovative methodology encompassing microfluidics based on fully automated chip-nanoelectrospray ionization (nanoESI), coupled with high-resolution mass spectrometry (MS) on a quadrupole time-of-flight (QTOF MS) instrument was developed. This platform was employed for the assessment of the noncovalent interactions between maltohexaose (Glc<sub>6</sub>) and β-lactoglobulin extracted from human milk upon deliberate intake of cow's milk. The experiments were carried out in (+) ESI mode, using ammonium acetate (pH 6.0) as the buffer and also in pure water. In both cases, the MS analysis revealed the formation of BLG-Glc<sub>6</sub> complex, which was characterized by top-down fragmentation in tandem MS (MS/MS) using collision-induced dissociation (CID). Our findings have a significant biomedical impact, indicating that Glc<sub>6</sub> binds BLG under conditions mimicking the in vivo environment and therefore might represent a ligand, able to reduce its allergenicity. Zamfir, Alina D. Chait, Brian T. Robu, Adrian C. 2016-02-18T08:51:30Z Schiopu, Catalin

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto