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Rkr1/Ltn1 Ubiquitin Ligase-mediated Degradation of Translationally Stalled Endoplasmic Reticulum Proteins

Rkr1/Ltn1 Ubiquitin Ligase-mediated Degradation of Translationally Stalled Endoplasmic Reticulum Proteins

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CROWDER, Justin J., Marco GEIGGES, Ryan T. GIBSON, Eric S. FULTS, Bryce W. BUCHANAN, Nadine SACHS, Andrea SCHINK, Stefan G. KREFT, Eric M. RUBENSTEIN, 2015. Rkr1/Ltn1 Ubiquitin Ligase-mediated Degradation of Translationally Stalled Endoplasmic Reticulum Proteins. In: Journal of Biological Chemistry. 290(30), pp. 18454-18466. ISSN 0021-9258. eISSN 1083-351X

@article{Crowder2015Rkr1/-32632, title={Rkr1/Ltn1 Ubiquitin Ligase-mediated Degradation of Translationally Stalled Endoplasmic Reticulum Proteins}, year={2015}, doi={10.1074/jbc.M115.663559}, number={30}, volume={290}, issn={0021-9258}, journal={Journal of Biological Chemistry}, pages={18454--18466}, author={Crowder, Justin J. and Geigges, Marco and Gibson, Ryan T. and Fults, Eric S. and Buchanan, Bryce W. and Sachs, Nadine and Schink, Andrea and Kreft, Stefan G. and Rubenstein, Eric M.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/32632"> <dc:contributor>Gibson, Ryan T.</dc:contributor> <dc:contributor>Buchanan, Bryce W.</dc:contributor> <dcterms:title>Rkr1/Ltn1 Ubiquitin Ligase-mediated Degradation of Translationally Stalled Endoplasmic Reticulum Proteins</dcterms:title> <dc:creator>Crowder, Justin J.</dc:creator> <dcterms:issued>2015</dcterms:issued> <dc:creator>Rubenstein, Eric M.</dc:creator> <dc:creator>Gibson, Ryan T.</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-01-19T15:01:06Z</dcterms:available> <dc:creator>Fults, Eric S.</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-01-19T15:01:06Z</dc:date> <dc:creator>Geigges, Marco</dc:creator> <dc:contributor>Kreft, Stefan G.</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/32632"/> <dc:contributor>Geigges, Marco</dc:contributor> <dc:contributor>Schink, Andrea</dc:contributor> <dc:contributor>Fults, Eric S.</dc:contributor> <dc:contributor>Crowder, Justin J.</dc:contributor> <dc:creator>Sachs, Nadine</dc:creator> <dc:contributor>Sachs, Nadine</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Schink, Andrea</dc:creator> <dcterms:abstract xml:lang="eng">Aberrant nonstop proteins arise from translation of mRNA molecules beyond the coding sequence into the 3'-untranslated region. If a stop codon is not encountered, translation continues into the poly(A) tail, resulting in C-terminal appendage of a polylysine tract and a terminally stalled ribosome. In Saccharomyces cerevisiae, the ubiquitin ligase Rkr1/Ltn1 has been implicated in the proteasomal degradation of soluble cytosolic nonstop and translationally stalled proteins. Rkr1 is essential for cellular fitness under conditions associated with increased prevalence of nonstop proteins. Mutation of the mammalian homolog causes significant neurological pathology, suggesting broad physiological significance of ribosome-associated quality control. It is not known whether and how soluble or transmembrane nonstop and translationally stalled proteins targeted to the endoplasmic reticulum (ER) are detected and degraded. We generated and characterized model soluble and transmembrane ER-targeted nonstop and translationally stalled proteins. We found that these proteins are indeed subject to proteasomal degradation. We tested three candidate ubiquitin ligases (Rkr1 and ER-associated Doa10 and Hrd1) for roles in regulating abundance of these proteins. Our results indicate that Rkr1 plays the primary role in targeting the tested model ER-targeted nonstop and translationally stalled proteins for degradation. These data expand the catalog of Rkr1 substrates and highlight a previously unappreciated role for this ubiquitin ligase at the ER membrane.</dcterms:abstract> <dc:creator>Kreft, Stefan G.</dc:creator> <dc:creator>Buchanan, Bryce W.</dc:creator> <dc:contributor>Rubenstein, Eric M.</dc:contributor> </rdf:Description> </rdf:RDF>

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